20S proteasome is a proteolytic complex committed to degrade unfolded proteins

The Ubiquitin-Proteasome System: The Cell's Natural Destroyer.

Identification of a Ubiquitin-Independent Degron by a Reporter Assay.

Experimental strategies to identify UID degrons that mediate degradation by the catalytic 20S proteasome particle are described and an integrated workflow-spanning degron mapping to functional validation-provides a generalizable framework for uncovering ubiquitin-independent degrons.

Dissecting PROTAB-mediated degradation of cell surface proteins.

It is shown that PROTAB induces rapid ternary complex formation, followed by receptor internalization and degradation, resulting in ~ 85% target depletion within 24 h, and mechanistically, ubiquitination enhances but is not strictly required for internalization, and degradation proceeds predominantly through the lysoso...

Midnolin-directed protein degradation beyond ubiquitin.

This review compares midnolin-directed degradation with proteolysis-targeting chimeras and molecular glue degraders and identifies the design, delivery, and safety principles that will determine whether it becomes a useful ubiquitin-independent complement to existing degraders.

Trans-branching of polyubiquitin chains orchestrates the DNA replication stress response

This study identifies hyper-accumulation of daughter-strand gaps as the mechanistic basis underlying the toxicity of inhibitors of the PCNA-specific isopeptidase, USP1, in BRCA1-deficient cells.

Activation and inactivation pathways of a p53-like transcription factor govern lipid homeostasis in yeast

The pathways that activate and inactivate Mga2 are defined, an evolutionary connection between the yeast lipid homeostasis regulator Mga2 and p53 is uncovered, and an evolutionary connection between the yeast lipid homeostasis regulator Mga2 and p53 is uncovered.