Enzyme Free Cloning proves an efficient and robust HTP cloning method, that is compatible with existing Ligation Independent Cloning vectors, and highly suitable for automation.
A streamlined cloning platform for expressing a gene of interest either alone or fused to a monomeric fluorescent protein under the control of two promoters of choice is described, providing a fast and flexible cloning strategy for protein expression and subcellular localization analyses in mycobacteria.
Maartje Deschutter, Eva Beaumale, S. Canaan et al.· Biochimie· 0 citations
A restriction enzyme-free and ligation-free PCR-based protocol widely applicable to any circular vector that enables targeted mutagenesis of the chikungunya virus (CHIKV) genome, offering a fast, efficient, and reliable strategy for generating mutant constructs suitable for virological and molecular studies.
N. T. Cerf, C. Filomatori· Methods in molecular biology· 0 citations
A nascent method for multiplex genome engineering in Saccharomyces cerevisiae that negates the requirement for cloning of expression cassettes is presented and the first reported use of intron-mediated assembly of promoters and ORFs for expression of a functional gene product is reported.
F. Harrison, Philip A. Kelso, Alexander C. Carpenter et al.· FEMS Yeast Research· 0 citations
Escherichia coli
-based expression systems are extensively utilized for recombinant protein production due to their simplicity, cost-effectiveness, and scalability. However, they often face challenges including leaky expression, codon bias, and poor solubility. Various strategies have been developed to address t...
Y. Chen, Jia-Lin Luo, Han Li et al.· Applied Microbiology and Bio...· 0 citations
A straightforward and reliable protocol for DNA isolation and storage in the screening of U. maydis mutants, enabling the reproducible amplification of 0.5-5 kb fragments directly from fresh colonies grown on agar plates.
Pascal Künzel, Andreas Müsgens, Lars M. Blank et al.· Methods in molecular biology· 0 citations
Beauveria bassiana is a filamentous fungus that has considerable potential to combat insect pests. Therefore, an easy to use genetic transformation tool helps to investigate which fungal genes play a role in virulence and host specificity. However, B. bassiana is often very resistant to antibiotics used as selective ag...
M. V. van Belkum, Anjalee Wijewardane McKee, John C. Vederas· Journal of Microbiological M...· 0 citations
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