Characterization of a novel CBM-independent GH13_5 raw starch-degrading α-amylase from Priestia koreensis HL12 for maltooligosaccharide production

Structural characterization and predicted biosynthetic pathway of the polysaccharide component of bioflocculant from starch-degrading Bacillus subtilis ZHX3.

Polysaccharides-based bioflocculant is a promising eco-friendly alternative to conventional flocculants, yet their application is limited by high production cost. Understanding the biosynthetic pathway is essential for targeted strain improvement. In this study, we characterized polysaccharides structure of biofloccula...

Influence of a Thermostable Fungal Xylanase Combined With Multi-Enzyme Complexes on the Structure, Physicochemical, and Functional Properties of Dietary Fiber From Hawthorn Pomace.

Effects of xylanase (XynA) in combination with various fibrolytic enzymes on the extraction of soluble dietary fiber (SDF) from hawthorn pomace and on the modification of dietary fiber (DF) were investigated. A high-yield fungal strain (Thermomyces lanugiosus Q231) producing a thermostable xylanase (XynA) was screened,...

A novel type III pullulan hydrolase from the bacterium Thermus scotoductus: Biochemical characterization and functional analysis of its carbohydrate-binding domains.

The results indicated that CBM48 was the major contributor to substrate binding and enzymatic activity, whereas CBM34 played a minor role in PulTS, which expands the knowledge of pullulan-hydrolyzing enzymes.

Identification and Biochemical Characterization of a Novel β-1, 3-glucanase for Efficient Preparation of Laminarin Oligosaccharides.

HPLC and ESI-MS analysis indicated that Cel2C can degrade laminarin and β-glucan into oligosaccharides with degrees of polymerization (DPs) of 1-7, and highlight the potential application of Cel2C as an endo-β-1, 3-glucanase in the biorefinery of laminarin biomass in related industries.

Protein profiling and GC–MS product analysis provide insights into lignite solubilization and bioconversion by Lysinibacillus sphaericus strain SH19

Genomic prospecting and biochemical characterization of a novel thermostable 3-quinuclidinone reductase from hot spring metagenomes for efficient biocatalysis

ABSTRACT This study presents the discovery and characterization of a novel thermophilic 3-quinuclidinone reductase (ScQR) identified through metagenomic mining of hot spring environments. ScQR, a member of the short-chain dehydrogenase/reductase (SDR) superfamily, was heterologously expressed in Escherichia coli, and i...