Jun 2026· Journal of Agricultural and Food Chemistry· Vol 74, pp. 20777-20784· 0 citations· 30 references
Medicine
TL;DR
It is suggested that palmitoleic acid may contribute to salt-stress adaptation in Z. rouxii by modulating energy and nucleotide turnover, while helping maintain redox balance and metabolic flexibility.
Abstract
Zygosaccharomyces rouxii is an important halotolerant yeast in high-salt fermentations, although the systems underlying its salt adaptation and nutritional regulation remain unknown. We characterized Z. rouxii CGMCC 3791 growing in YPD under control conditions or 120 g/L NaCl and assessed palmitoleic acid supplementation during salt stress. DIA-based LC-MS/MS proteomics and UPLC-Orbitrap metabolomics were combined with KEGG enrichment and pathway mapping. PCA effectively differentiated the three groups across both omics layers, demonstrating that palmitoleic acid induces a unique reprogramming beyond the salt-only response. The enrichment analysis revealed the coordinated regulation of amino acid metabolism, glutathione-associated redox activities, ABC transporters, oxidative phosphorylation, and cofactor pathways, including pantothenate and CoA biosynthesis. Integrated mapping demonstrated increased purine-related intermediates (FAICAR, IMP, ADP, and inosine) and lipid-related remodeling. Overall, these results suggest that palmitoleic acid may contribute to salt-stress adaptation in Z. rouxii by modulating energy and nucleotide turnover, while helping maintain redox balance and metabolic flexibility.
Under severe stress, GO and KEGG enrichment consistently revealed the reinforcement of central carbon and energy metabolism, peroxisome-associated fatty acid turnover, oxidoreductase/redox activities, and translation and nucleotide metabolic pathways.
Dingkang Wang, Li Wang, Yue Xiao et al.· Journal of Proteome Research· 0 citations
Deciphering the metabolic basis of high-yield antibiotic production in Streptomyces is crucial for strain optimization. Atmospheric and room-temperature plasma (ARTP) mutagenesis of Streptomyces xinghaiensis sf106 generated a mutant with a 30% increase in tylosin-equivalent concentration (μg/mL). 4D-FastDIA quantitative proteomics identified 279 differentially abundant proteins enriched in the Type I polyketide synthase (PKS) pathway, with increased abundance of key macrolide-biosynthesis-related proteins. Lysine-acetylome profiling identified 1152 differentially abundant acetylation sites and revealed altered acetylation of enzymes involved in fatty acid metabolism and the tricarboxylic acid (TCA) cycle, suggesting adjustments in central metabolism associated with acyl-CoA precursor availability and energy generation. Integration of proteomic and acetylomic data suggests coordinated changes in protein abundance and lysine acetylation associated with the increased tylosin-equivalent concentration. These results highlight candidate nodes for rational metabolic engineering of S. xinghaiensis.
C. Jia, Cilang Ma, Yuting Jiang et al.· Microbiology Research· 0 citations
Temperature constrains stable mycelial growth and production of Oudemansiella raphanipes, but its molecular response to heat stress followed by recovery remains insufficiently resolved. We integrated untargeted LC-MS metabolomics and RNA sequencing to compare control mycelia maintained at 28 °C (HPJZ28) with mycelia exposed to 42 °C for 6 h and then allowed to recover at 28 °C for 2 h (HPJZ42-R). Metabolomic and transcriptomic profiles separated clearly between the two conditions, indicating broad post-heat recovery-associated molecular remodeling. Most differential metabolites were lower in HPJZ42-R, whereas a smaller subset accumulated, suggesting selective metabolic reorganization rather than generalized activation. Transcriptome analysis identified extensive gene-expression remodeling, with 1081 upregulated and 1878 downregulated genes in the HPJZ28 versus HPJZ42-R comparison. Pathway-level analyses implicated central carbon metabolism, lipid metabolism, amino acid metabolism, peroxisome-related processes, and calcium signaling. Because the sampling design included a recovery period and a single post-stress time point, integrated gene–metabolite correlations are interpreted as exploratory associations rather than evidence of direct regulatory coupling. These results provide species-level multi-omics evidence for the post-heat recovery state of O. raphanipes and identify candidate pathways for future functional and physiological validation.
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Results demonstrate that salinity tolerance in rice is associated with coordinated transcriptional and metabolic reprogramming that supports oxidative stress mitigation and adaptive stress responses.
Chander Kant Chaudhary, P. Guttula, Kirti Agrawal et al.· bioRxiv· 0 citations
Paracoccidioidomycosis (PCM) is a neglected systemic mycosis whose etiologic agents must adapt to acidic host niches such as phagolysosomes. Here, we used quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics to define the response of Paracoccidioides brasiliensis to acidic stress (pH 4.5) versus control pH (6.5) after 5 and 24 h. We identified and quantified 4374 proteins, including 327 and 722 differentially abundant proteins at 5 and 24 h, respectively, revealing time-dependent proteomic reprogramming. Enrichment analyses highlighted proteolysis, protein metabolism, organonitrogen metabolism, MAPK- and SNF1-like signaling, central carbon metabolism, tyrosine metabolism, and ergosterol biosynthesis as major acid-responsive processes. Complementary assays showed pH-dependent extracellular proteolytic activity, increased adhesion to A549 pulmonary epithelial cells, and dynamic ergosterol remodeling. The proteomic data further indicated increased abundance of moonlighting proteins linked to adhesion and metabolic enzymes associated with ATP generation and melanin precursor production. Together, these findings indicate that P. brasiliensis adapts to acidic environments through coordinated regulation of proteostasis, metabolism, signaling, host-cell interaction, and membrane homeostasis, supporting survival and virulence potential in acidic host microenvironments.
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Combined stress more strongly inhibited plant height, stem diameter, fresh weight, net photosynthetic rate, and transpiration rate than single stresses, and insights into alfalfa adaptation to multiple abiotic stresses are provided.
Lihe Su, Yongcheng Chen, Xudong Zhang et al.· Journal of Agricultural and...· 0 citations
