Apo and substrate-bound dihydropteroate synthase crystal structures from Thermus thermophilus HB8.

Effect of Structure-Guided Single Mutant on Nicotinamide Coenzyme Specificity of Glucose Dehydrogenase from Bacillus Megaterium

A structure-guided rational design to invert the coenzyme specificity of GDH by targeting a single residue within the conserved GXXXGXG motif of the Rossmann fold provides a generalizable framework for engineering cofactor preference in short-chain dehydrogenase/reductase family enzymes.

Structural insights into sulfonamide degradation by a two-component flavin-dependent monooxygenase.

The sulfonamide-degrading monooxygenase sulX plays a dual role in bioremediation and antibiotic resistance, yet its molecular mechanism remains elusive. Here we report crystal structures of sulX in its ligand-free form, as an FMN-bound binary complex, and as ternary complexes with six distinct sulfonamides at resolutio...

Structural basis for substrate recognition in l-lysine 6-dehydrogenase from Geobacillus stearothermophilus by Cryo-EM.

l-lysine 6-dehydrogenase (LysDH; EC 1.4.1.18) oxidatively deaminates the ε-amino group of l-lysine. Due to its high substrate specificity, LysDH serves as a valuable tool for l-lysine quantification. However, the molecular basis of this specificity has remained unclear because of the lack of substrate-bound structures....

Characterization of NADH-insensitive phosphoribulokinase from a marine, obligately chemolithoautotrophic hydrogen- and sulfur-oxidizing bacterium Hydrogenovibrio marinus MH-110.

Analysis of the PRK activity in the cell-free extract of H. marinus and the purified recombinant HmPRK (rHmPRK) expressed in Escherichia coli revealed the activity as unaffected by NADH, indicating that HmPRK has unique properties distinct from those of the canonical class III PRKs.

[Cofactor preference engineering of meso-diaminopimelate dehydrogenase and its application in d-phenylalanine synthesis].

Isoprenoid Binding and Substrate Channeling in Drimenol Synthase, a Bifunctional Class II Terpene Cyclase-Phosphatase

More than one thousand bifunctional terpene synthases combining prenyltransferase and terpene cyclase activities have been identified in bacteria and fungi, but only a handful of enzymes have been identified that combine terpene cyclase activity with a downstream processing activity. Drimenol synthase from the marine b...