The present review discusses the use of CRISPR-Cas genome editing technologies as an accurate and effective approach to increasing chilling tolerance in rice, and establishes a conceptual framework to overcome translational challenges in CRISPR-mediated improvement of complex traits in oilseed crops.
Overall, CRISPR-Cas-based genome editing represents a promising and efficient approach for accelerating the development of high-yielding, climate-resilient, and stress-tolerant rice cultivars, thereby contributing significantly to sustainable rice production and global food security under changing environmental conditions.
Sravani Verupanda, A. Chakraborty, Mimansha Shrivastava et al.· Journal of Applied Genetics· 0 citations
CRISPR/Cas-based genome editing has emerged as a powerful and precise tool for crop improvement, enabling targeted modification of genes associated with agriculturally important traits. In horticultural crops, CRISPR technologies have accelerated the improvement of disease resistance, abiotic stress tolerance, yield, nutritional quality, shelf life, flowering behavior, and ornamental characteristics. Among available genome-editing platforms, CRISPR/Cas9 is the most widely utilized because of its simplicity, efficiency, and versatility. The technology enables precise genome modification through targeted DNA cleavage followed by endogenous repair mechanisms, facilitating gene knockout, insertion, or sequence alteration. Recent advances in genome editing have significantly expanded its applications in vegetable, fruit, and ornamental crops. Successful modifications targeting genes associated with stress tolerance, fruit ripening, pigment biosynthesis, flowering regulation, and pathogen resistance demonstrate the enormous potential of CRISPR-mediated breeding for horticultural improvement. However, several challenges, including low transformation efficiency, genotype-dependent regeneration, prolonged juvenile phases, polyploidy, and regulatory concerns, continue to limit its broader application in many horticultural species. This review summarizes recent progress in CRISPR/Cas-mediated genome editing in horticultural crops, including strategies for guide RNA design, transformation, regeneration, development of transgene-free plants, and regulatory considerations. Furthermore, emerging advances such as precision editing technologies and improved delivery systems are discussed as promising approaches for enhancing editing efficiency and expanding future applications. Overall, CRISPR/Cas technologies hold substantial potential for accelerating the development of climate-resilient, high-quality, and nutritionally improved horticultural crops.
Prerna Srivastava, D. Singh, Rima Kumari et al.· Discover Plants· 0 citations
This review systematically examines how CRISPR-Cas9 enables targeted engineering of stress tolerance in major crops through gene knockout and knock-in strategies, and highlights emerging synergies with functional genomics, multi-omics integration, and high-throughput phenotyping to accelerate target discovery and validation.
T. Khan, A. A. Abro, U. Zulfiqar et al.· Functional & Integrative Gen...· 0 citations
This review provides a comprehensive synthesis of a recent advances in CRISPR–Cas technologies and their strategic applications in crop genetics and hybrid breeding, and showcases how these technologies accelerate hybrid breeding by engineering male sterility systems, fixing heterosis, and generating high-throughput mutant libraries for trait discovery.
Syed Riaz Ahmed, Jahangir Khan, I. Ijaz et al.· Frontiers in Plant Science· 0 citations
Overall, the evidence suggests that CRISPR Cas-9 has the potential to accelerate plant breeding, improve food security, and support the development of climate resilient crops while reducing the time and uncertainty associated with traditional breeding methods.
Victoria Imafidor· World Journal of Advanced En...· 0 citations
The results demonstrate the successful deployment of CRISPR/Cas9 for targeted genome engineering in sugarbeet and establish a reliable platform for future gene-editing efforts aimed at enhancing resistance to a wide range of pathogens and diseases affecting the crop.
Z. Khan, Tinley Hathaway, C. Chu et al.· Frontiers in Genome Editing· 0 citations