An Overview of Mass Spectrometry-Based Methods to Analyze Post-Translational Modifications.

Identification of Protein Cysteine Modifications Using Unbiased Mass Spectrometry-Based Proteomics.

STEP-PTMs: Sequential TMT-based Enrichment and Profiling of Post-Translational Modifications

Application of STEP-PTM to TMT16-plex cerebral brain organoids enabled the quantification of 10,413 proteins, 2,969 metabolites, 19,655 phosphopeptides, 28,876 peptides containing reversibly modified cysteines, 9,723 peptides containing free cysteines, 1,716 intact sialylated N-linked glycopeptides and 771 lysine-acety...

BeyondTACs: Chemical Editing of Posttranslational Modifications (PTMs) for Therapeutic Intervention.

Protein posttranslational modifications (PTMs) build up the diversity and complexity of the proteome, and their dysregulation is intrinsically linked to various diseases. The study of PTMs has profoundly broadened the scope of therapeutic target discovery. Building upon the success of PROTACs, this heterobifunctional p...

Emerging Roles of Mass Spectrometry-Based Proteomics in Drug Discovery.

Mass spectrometry (MS)-based proteomics has emerged as a versatile platform in modern drug discovery, enabling system-wide interrogation of protein abundance, posttranslational modifications (PTMs), turnover, structural stability, and interactions with small molecules in native biological contexts. In this review, we i...

Post-translational modifications in bacteria: From identification to function.

Post-translational modifications (PTMs) provide molecular variety beyond the gene or amino acid codes. Diversity stems from a vast number of chemical species or physical changes that are regulated either enzymatically or non-enzymatically to provide transient or prolonged alterations to protein structure and function....

Proteoforms in Disease: Biomedical Applications of Top-Down Proteomics.

The evolving role of TDP in biomedical research is reviewed, highlighting studies that revealed proteoform-level alterations, identified candidate biomarkers, and advanced the understanding of the roles of proteoforms in human diseases.