Open access

STEP-PTMs: Sequential TMT-based Enrichment and Profiling of Post-Translational Modifications

Lucrezia Criscuolo S. B. Elmkvist Arkadiusz Nawrocki L. Jakobsen Pia Jensen P. T. Jensen Honggang Huang J. Havelund N. Færgeman G. Palmisano H. Bogetofte M. R. Larsen

Aug 2026 · bioRxiv · 0 citations

Biology

TL;DR

Application of STEP-PTM to TMT16-plex cerebral brain organoids enabled the quantification of 10,413 proteins, 2,969 metabolites, 19,655 phosphopeptides, 28,876 peptides containing reversibly modified cysteines, 9,723 peptides containing free cysteines, 1,716 intact sialylated N-linked glycopeptides and 771 lysine-acetylated peptides from the same biological samples.

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