Generation of High-Quality Succinyl Spectral Libraries for Improved Proteome-Wide Succinylome Analysis Using Data-Independent Acquisition.

Alice Zhang B. Schilling J. Bons

2026 · Methods in molecular biology · Vol 3018, pp. 169-181 · 0 citations

Medicine

TL;DR

How to build DDA PTM spectral libraries using various software tools, specifically Spectronaut, SpectroMine, and MSFragger are described, which were imported into Skyline for the analysis of previously published DIA succinylome data of Sirtuin-5 knocked-out vs wild-type mouse brains.

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