AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
TL;DR
The combination of SDS-PAGE-based protein fractionation with CZE-tandem MS (MS/MS) for high-resolution characterization of histone proteoforms from a commercial calf thymus histone sample was described.
Top-down proteomics (TDP) enables direct characterization of intact proteoforms, providing protein-level insights into molecular diversity arising from post-translational modifications and sequence variations. Despite this advantage, proteome coverage in TDP remains limited relative to bottom-up proteomics (BUP). To ex...
Capillary electrophoresis-mass spectrometry (CE-MS) is a promising technique for ultrasensitive top-down proteomics due to its high resolution and sensitivity compared with traditional liquid chromatography (LC)-based methods. However, CE-MS-based top-down proteomics of mass-limited samples remains challenging becaus...
Protein complexes can be modeled with data arising from crosslinking mass spectrometry, but it is difficult to collect such data from cellular sources, particularly for proteins that are part of extensive interaction networks. Here, we present a tandem-affinity purification crosslinking mass spectrometry (xTAP‑MS) meth...
Alteration of proteins by attaching lipid moieties is a type of post-translational modification that affects the properties of proteins. It plays a role in various physiological processes, mostly due to direct interaction with cell membranes. Proteomic identification of lipoproteins typically depends on detecting their...
This review summarizes the evolution, principles, workflows, strengths, limitations, and representative applications of MS, SomaScan, Olink PEA, and Nautilus Voyager, and discusses their integration in biomarker discovery, population proteomics, and precision medicine.
Mass spectrometry (MS)-based nucleic acid analysis provides direct chemical evidence for oligonucleotide sequence, composition, and modifications. However, oligonucleotide LC-MS analysis commonly relies on ion-pairing reversed-phase liquid chromatography (IP-RPLC). Although IP-RPLC provides strong retention and high-re...
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