The Role of Cold Shock Protein (CSP) Gene Family in Yak (Bos grunniens) for High-Altitude Adaptation: Genome-Wide Identification and Analysis.

Unveiling the CpHsp and CpHsf Gene families in the zucchini genome: Evolution, structure, and stress-responsive expression

The key marker genes and in silico interaction networks identified herein provide a robust resource for functional characterization and molecular breeding to improve stress resilience in zucchini.

Genome-wide characterization of heat shock protein genes reveals thermal stress-responsive candidates in Litopenaeus vannamei.

A genome-wide analysis of the HSP gene family is performed to provide a genomic framework for HSP genes in L. vannamei and identify candidate genes and variants associated with thermal stress responses.

Genome-wide characterization highlights the regulatory network rewiring of BTB genes in barley under stress condition

Genome-Wide Characterization and Reproductive Tissue-Specific Heat-Responsive Expression Analysis of the HSP90 Gene Family in Common Bean (Phaseolus Vulgaris L.)

The results indicate that PvHsp90 genes are evolutionarily conserved and differentially regulated during heat stress, highlighting PvHsp90‑1 and PvHsp90‑2 as promising candidates for improving reproductive thermotolerance and heat resilience in common bean.

GENOME-WIDE ANALYSIS AND IDENTIFICATION OF HSF (HEAT SHOCK TRANSCRIPTION FACTOR) GENES IN COFFEE (COFFEE ARABICA L.) UNDER ABIOTIC STRESS

Coffee is a livelihood source for millions of farmers and plays a significant role in the economy of many coffee-producing countries. Abiotic stresses, such as drought and high temperature, however, negatively impact coffee growth, productivity, and bean quality. Heat shock factors (HSFs) play a crucial role in the plant response to heat and other abiotic stresses. However, there is still not a lot of detailed knowledge about the HSF gene family in Coffea arabica. In this study, a genome-wide search was performed to find and characterize the genes of HSF from C.arabica. A total of 59 putative CaHSF genes have been identified and characterized according to their physicochemical properties, chromosomal distribution, gene structure, conserved motifs, phylogenetic relationship, duplication event, synteny, cis-regulatory elements, and protein–protein interactions. The genes identified for CaHSF were assigned to different scaffolds of the genome, and they had different sizes, molecular mass, isoelectric point, GRAVY value, and aliphatic index of their protein. Through the relationships induced by the phylogenetic and conserved-domain analyses, and by their conservation, it was possible to get insight into the evolution of the proteins of the CaHSF family. Abiotic stress response, hormone signaling, and light regulation were noted to have several cis-regulatory elements associated with them in the promoter analysis and hence the possible role of the CaHSF genes in stress adaptation. Duplication and synteny analyses also showed that gene duplication played a role in the diversification and expansion of the CaHSF gene family in C. arabica. Overall, this study is a thorough genomic analysis of the CaHSF gene family, which can serve as a basis for future functional studies for understanding the molecular mechanism of abiotic stress tolerance in coffee.

Genome-wide identification of the SnRK2 gene family and its response to drought stress in Bombax ceiba

Introduction Drought is a major abiotic stress limiting the growth and ecological adaptation of tropical and subtropical trees. The SnRK2 gene family is a core regulator in ABA signaling and drought response pathways. However, genome-wide identification and functional characterization of the SnRK2 family remain unclear in Bombax ceiba, a typical drought-tolerant tropical pioneer tree species with important ecological and economic value. Methods We performed genome-wide identification of the BcSnRK2 gene family using bioinformatics approaches. Phylogenetic relationships, gene structures, conserved motifs, cis-acting elements, chromosomal localization, and protein structures were systematically analyzed. Subcellular localization was verified by transient expression in Nicotiana benthamiana. Tissue-specific expression and drought-responsive patterns were detected by qRT-PCR under 10% PEG6000 treatment. Protein–protein interaction networks were predicted using the STRING database. Results A total of nine BcSnRK2 genes were identified and unevenly distributed across eight chromosomes. All BcSnRK2 proteins contained conserved kinase domains and shared a highly conserved exon–intron structure. Promoter regions harbored abundant ABA-responsive and stress-related cis-elements. BcSnRK2 genes exhibited distinct tissue-specific expression profiles. All genes were significantly induced by drought stress in a tissue- and time-dependent manner, with BcSnRK2.9 and BcSnRK2.7 showing strong and sustained activation in shoots and BcSnRK2.7 and BcSnRK2.3 responding prominently in roots. BcSnRK2 proteins were localized in the cytoplasm, plasma membrane, and nucleus, and were predicted to interact with core components of the ABA signaling pathway. Discussion The BcSnRK2 family exhibits evolutionary conservation and functional divergence in Bombax ceiba. The compact size of the SnRK2 family, conserved structural features, and distinct tissue-specific drought response patterns are consistent with a streamlined stress signaling system that may contribute to the ecological adaptation of Bombax ceiba in seasonally dry tropical environments, although formal evolutionary analyses are required to establish adaptive significance. This study provides valuable gene resources for drought resistance breeding of woody plants and advances the understanding of stress signaling mechanisms in tropical trees.