Findings identify a candidate insertion site for efficient expression of foreign antigens in the PEDV genome and provide a technical basis for the development of PEDV-based viral vector platforms.
Abstract Usutu virus (USUV) is an emerging mosquito-borne orthoflavivirus closely related to West Nile and Japanese encephalitis viruses. Despite its increasing prevalence in Europe and reports of neuroinvasive disease in humans, the molecular and immunological determinants of USUV infection remain poorly understood, l...
Amy N. Nelson, Aaron E. Lin, Saloni Sinha et al.· PNAS Nexus· 0 citations
ABSTRACT Reporter-expressing recombinant influenza A viruses (IAVs) have emerged as powerful tools for studying viral infection, host–pathogen interactions, and the identification and characterization of prophylactic and/or therapeutic countermeasures for the treatment of IAV infections. By incorporating a reporter gen...
Ramya S. Barre, H. Nath, A. Nogales et al.· Journal of Virology· 0 citations
Bovine viral diarrhea virus type 1 (BVDV-1) is an RNA virus known for lifelong persistent infection in affected animals and substantial economic impact in cattle. While current approaches of infection control rely on biosecurity and immunization, recent advances in genome editing provide opportunities for improving gen...
Christian W Eaton, Aspen M. Workman, J. Loy et al.· Journal of Animal Science· 0 citations
Newcastle disease virus (NDV) remains a major threat to poultry production worldwide, requiring reliable molecular diagnostic tools. In this study, a recombinant plasmid carrying the matrix (M) gene of NDV was constructed and characterized for potential use as a positive control in PCR-based diagnostic systems. Viral R...
N. Ibraim, F. Ikramkulova, M. Zheney et al.· МИКРОБИОЛОГИЯ ЖӘНЕ ВИРУСОЛОГ...· 0 citations
African swine fever virus (ASFV) is a highly pathogenic agent that poses a severe threat to the global swine industry. The biological function of A859L, an ASFV-encoded RNA helicase, remains largely elusive. Here, we show that A859L is highly conserved across diverse ASFV isolates and belongs to the SF2 superfamily of...