Cell motility is essential for processes such as immune responses, wound healing, and fertilization. Caenorhabditis elegans is an excellent model for studying sperm motility, due to the worm's transparency and high sperm production. C. elegans amoeboid sperm move by extending and retracting pseudopods, which are powere...
Jenna K. Jablonski, Olivia D. Warren, Elena B. Benson et al.· Developmental Biology· 0 citations
Barrier epithelia are shielded from the external environment by their apical extracellular matrices (aECMs). The molecular complexity of aECMs has challenged understanding of their organization in vivo. To define the molecular architecture of a model aECM we generated a toolkit of 105 fluorescently tagged aECM componen...
J. M. Ragle, Murugesan Pooranachithra, Guinevere A. Ashley et al.· PLoS Biology· 0 citations
These data provide a configuration for driving nuclear and secreted proteins from a single-copy transgene and indicate that downstream secreted proteins could be correctly directed to the secretory pathway.
J. M. Ragle, Guinevere A. Ashley, Jordan D. Ward· microPublication Biology· 0 citations
This work describes how to generate degron knock-ins by CRISPR, cross them to TIR1-expressing strains, and perform depletion experiments, and provides a description of the current methods and highlight alternative approaches.
Jordan D. Ward· Methods in molecular biology· 0 citations
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