Protocol for robust gene knockout and reliable validation in human cell lines using quad-guide RNA vectors
This strategy provides an efficient workflow for gene knockout that is rapidly confirmed through PCR amplification of mRNA derived from the targeted gene loci that is rapidly confirmed through PCR amplification of mRNA derived from the targeted gene loci.
Michael Tobin, Jie Dai, Li-Chuan Chan et al.
· STAR Protocols · 0 citations