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Author

Shinya Fushinobu

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4 papers indexed here

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Sep 2026

Elucidation of D-alanine biosynthetic pathways in the hyperthermophiles: Characterization of alanine racemase from Thermus thermophilus and the structure and dual physiological functions of lysine racemase from Thermotoga maritima.

The hyperthermophilic bacterium Thermotoga maritima contains a peptidoglycan comprising D-alanine, D-glutamate, and D-lysine. We have previously identified the biosynthetic pathways of D-lysine and D-glutamate; however, the biosynthetic pathway of D-alanine is unclear in T. maritima. The present study elucidated the D-...

Tetsuya Miyamoto, T. Moriya, Shunpei Nitta et al. · 0 citations
Open access Sep 2026

GH172 Difructose Dianhydride I Synthase: A Two-Stepped Journey via Glycosylation and Cyclization Using QM/MM Metadynamics.

The difructose dianhydride I synthase/hydrolase α FFase 1 catalyzes the reversible transformation of inulobiose into difructose dianhydride I, a component of caramel. The proposed reaction mechanism of such a transformation consists of a glycosylation step followed by a cyclization to yield the dianhydride from the dis...

Santiago Alonso-Gil, Bruno Di Geronimo, T. Kashima et al. · 0 citations
Open access Sep 2026

Structural mechanism defining product specificity in glycoside hydrolase family 66 cycloisomaltotetraose glucanotransferase

The crystal structures of CI4Tase are determined and the strict size-control mechanism of CI4Tase is elucidated, providing a structural foundation for engineering cycloisomaltooligosaccharide-producing enzymes with optimized transglycosylation efficiency and specificity for industrial applications.

Ryota Yasukochi, T. Kashima, Tetsuya Mori et al. · 0 citations
#protein folding Open access Sep 2026

Crystal structure of a class II UDP-glucose—hexose-1-phosphate uridylyltransferase from Bifidobacterium longum involved in human milk oligosaccharide metabolism

Infant-associated bifidobacteria utilize lacto-N-biose I (LNB) and galacto-N-biose (GNB), major components of human milk oligosaccharides and intestinal mucin, respectively, through the GNB/LNB metabolic pathway. A UDP-glucose–hexose-1-phosphate uridylyltransferase (GalT) from Bifidobacterium longum JCM 1217 (BlGalT2)...

M. Sato, C. Yamada, M. Nishimoto et al. · 0 citations

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