gene editing

Comparative mitogenomics of Roscoea alpina and R. purpurea from contrasting elevations reveals mitochondrial genomic differentiation

Plant mitochondrial genomes are structurally dynamic and contribute to energy metabolism and stress responses. Comparative mitogenomic analyses of closely related species occupying contrasting elevations can provide insights into mitochondrial genomic differentiation across elevational environments. Using PacBio HiFi r...

Knockout of SlERF.F4 transcription factor enhances drought resilience in tomato

Since plants are constantly exposed to ever-changing climate conditions, they evolved an intricate system that perceives environmental stresses and integrates them in order to adapt their response. At a molecular level, transcription factors play an important role in regulating the plant’s response by influencing e...

Genome editing beyond transgenes: DNA-free technologies in crop improvement

Conventional and transgenic approaches to crop improvement are increasingly constrained by biosafety concerns, stringent regulatory frameworks, and limited public acceptance, necessitating new precision breeding methods. DNA-free genome editing using the CRISPR/Cas9 approach has emerged as a transformative approach t...

Synthetic Dataset for PRIVAGEN-SHIELD: Privacy-Preserving Genomic Anomaly Detection

Dataset Description This repository contains the synthetic genomic workflow dataset generated and used for the experimental evaluation of the PRIVAGEN-SHIELD framework, “PRIVAGEN-SHIELD: Privacy-Preserving Genomic Anomaly Detection via Hierarchical Blockchain-Authenticated Merkle Trees and Local Differential Privacy.”...

CropStabDB: a protein stability resource and Engineering Atlas of orthologue-supported substitutions for 30 plant proteomes

Protein stability, the capacity to maintain a folded structure and function, matters as much for transporters and regulators as for enzymes, and heat erodes it. Wheat, rice, maize and soybean supply two-thirds of human caloric intake, and warming reduces yields. Rubisco activase variants selected for thermostability in...

Functional divergence of TLR5 paralogs reveals distinct roles for TLR5M and TLR5S in salmonid Immunity.

Sensing of pathogen-associated molecular patterns (PAMPs) is a critical step in mounting effective immune responses against invading pathogens. Toll-like receptor 5 (TLR5) is central to this process through its recognition of bacterial flagellin. While the function of the canonical membrane-bound form (TLR5M) appears c...

Redirecting full-length FLT1 expression towards its soluble isoform by targeting alternative polyadenylation promotes postischaemic angiogenesis.

Using this mouse model, it is demonstrated that sFLT1 alone is sufficient for developmental vasculogenesis, whereas the specific loss of flFLT1 enhances reparative revascularization and angiogenesis and improves cardiac recovery after myocardial infarction.

Progress in polymeric nanoparticle-based treatment approaches for Wnt β-catenin pathway modulation and adenomatous polyposis coli mutant colorectal cancer.

About 80-90% of sporadic instances of colorectal cancer (CRC) are caused by mutations in the adenomatous polyposis coli (APC) gene, which is one of the earliest and most common molecular events. The constitutive activation of the Wnt/β-catenin signalling pathway caused by APC dysfunction promotes unchecked cellular pro...

Molecular dynamics simulation and QM/MM study of IscB-ωRNA complex.

The CRISPR-Cas9 system's gene-editing fidelity relies on the HNH nuclease domain's ability to hydrolyze DNA via metal-ion catalysis. While IscB-ωRNA variants offer advantages for viral delivery due to their small size, their editing efficiency is much lower than that of Cas9. The understanding of how HNH-domain dynamic...

Are We Measuring Scientific Intelligence? Rethinking the Evaluation of AI Scientists

AI agents can now carry out data-driven scientific analyses end to end, and benchmarks assess them by giving an agent a question and a dataset and scoring its final answer against a fixed key. These benchmarks assume that a correct answer was derived from the supplied data, a property we call evidence grounding. Howeve...

Anthropic's discovery will, at best, be just another gene-editing tool

Programs and models for incentivizing biotechnology development in medical manufacturing: foreign experience

The presented international experience can establish a foundation for an effective response to the main challenges hindering biotechnology development in Russia, however, achieving maximum efficacy will require adaptation of these tools to the specific economic and legal realities of the Russian Federation.