gene editing

Alfalfa-mediated phytoremediation and soil restoration for sustainable cereal and fruit crop production: a review of roles, detoxification mechanisms, nitrogen enhancement, and agroecological integration

Soil contamination by heavy metals, petroleum hydrocarbons, and excessive agrochemical inputs, coupled with declining soil fertility, poses a major threat to sustainable cereal and fruit production and global food security. This review synthesizes evidence from peer-reviewed studies published between 2000 and 2026 to...

Waterlogging tolerance in pigeonpea: current understanding of physiological responses, genetic resources, and genomic prospects

An evidence-based framework provides a roadmap for moving from observed tolerance mechanisms toward experimentally validated molecular targets and their deployment in climate-resilient pigeonpea breeding.

Pathway-wide base editing charts chemical-genetic interactions in MAPK signaling

These findings establish BE scanning as a scalable approach for network-level chemical-genetic interaction mapping, revealing new mechanistic insights into one of the most studied oncogenic pathways.

Therapeutic upregulation of gene expression in inherited cardiomyopathies from current approaches to future directions

The mechanisms and current uses of gene upregulation strategies are reviewed and their advantages and limitations are critically evaluated as therapies for inherited cardiomyopathies.

Defending synthetic DNA orders against intra-order splitting-based obfuscation

Biosecurity screening of synthetic DNA orders is a key defense against malicious actors and careless enthusiasts producing dangerous pathogens or toxins. It is important to evaluate biosecurity screening tools for potential vulnerabilities and to work responsibly with providers to ensure that vulnerabilities can be pat...

Advances in plant metabolic engineering through synthetic biology

This review highlights the primary technologies used in plant metabolic engineering and focuses on representative examples of their implementation for crop improvement, including but not limited to CRISPR-Cas systems, multiplex genome editing, knock-in and knock-out strategies, CRISPR-mediated gene regulation, base edi...

Phytomedicinal approaches to postprandial hyperglycemia: Plants with α‑amylase and α‑glucosidase inhibitory effects (Review)

Diabetes mellitus (DM), a non-communicable disease with a high disease burden, is characterized by insulin resistance and postprandial hyperglycemia.An estimated 589 million adults worldwide were suffering from DM in 2024, a number projected to exceed 853 million by the year 2050.Diabetes has an evolving epidemiologica...

Wheat genetic resources for sodicity tolerance: from salinity-derived evidence toward sodicity-specific breeding

Disease Modeling of Fabry Disease Cardiomyopathy using Base Editing-Established Isogenic iPSCs and Application of Prime Editing for Gene Correction

Generation of Human Cells with Monoallelic Expression of HLA‑A in HLA Class I Knockout Cells for Evaluation of Antigen Presentation

Neu5Gc and beyond: sialylation at the innate immune and thromboinflammatory interface of xenotransplantation

Recent advances in genetic engineering have substantially reduced the major carbohydrate barriers to pig-to-human xenotransplantation. Deletion of GGTA1, CMAH, and B4GALNT2 decreases αGal, Neu5Gc, and B4GALNT2-dependent Sda-like xenoantigens and thereby attenuates preformed antibody binding and complement-mediated inju...

A photostable version of HY5 confers tolerance to proximity shade and improved defense responses in tomato

The results indicate that the editing approach allows the generation of gain-of-function tomato plants in which HY5 is camouflaged to avoid COP1 recognition and eventual degradation, and provides a biotechnological tool to create more compact and pathogen-resistant plants amenable to high planting densities.