gene editing

Artificially Engineered Carcinogenesis as a Biosecurity Threat: A Policy Review and Call for Inclusion in National and International Governance Frameworks

This paper calls for the explicit recognition of artificially engineered carcinogenesis as a potential biosecurity threat and proposes targeted policy measures to strengthen governance, including risk assessment frameworks, enhanced ethical review processes, and integration of oncogenic risk considerations into biosecu...

Stoichiometric m6A regulation of YY1 orchestrates the metabolic switch during cardiac development

Chemical modifications of RNA provide an additional regulatory layer essential for development. N6A methyladenosine (m6A) is the most abundant internal mRNA mark, but how site specificity, stoichiometry, and cellular context together determine mRNA fate remains unclear. Here, we define these rules in cardiogenesis by c...

The conserved function of bHLH121 and overexpression of N-terminal bHLH121 fragment suppresses iron deficiency response in Arabidopsis

Iron (Fe) homeostasis is fundamental to plant growth and development and is centrally regulated by a well-conserved network of basic helix-loop-helix (bHLH) transcription factors. In Arabidopsis thaliana, bHLH121 regulates the expression of FER-like iron deficiency-induced transcription factor (FIT) and clade Ib bHLH38...

Cargo size matters when designing lipid nanoparticles for delivery of large gene editors

Endoplasmic reticulum-associated NcDYRK2 regulates Ca2⁺ dynamics, motility, and development gene expression in Neospora caninum

Neospora caninum is an obligate intracellular apicomplexan parasite and a leading cause of bovine abortion worldwide, imposing substantial economic losses on the cattle industry. Dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs) are highly conserved serine/threonine kinases that regulate diverse cellu...

Probing the Conformational Dynamics of VeCas9 Using High-Resolution Single-Molecule Junctions.

By integrating VeCas9 into a graphene-molecule-graphene single-molecule junction platform, mechanistic insights into the Cas9 function are provided and the strong potential of high temporal-resolution single-molecule techniques for studying dynamic biological processes is demonstrated.

Understanding the genetic and epigenetic code of osteoporosis: Integrating the central role of Sirtuins in skeletal ageing

Hormone-sparing non-surgical male contraception in dogs and cats: Current strategies and the emerging role of FSH receptor-targeted antibody fragments.

Current non-surgical male contraceptive approaches in dogs and cats are summarized, including GnRH agonists, progestogens, androgens, systemic chemical contraceptive agents, intratesticular and intraepididymal chemical contraceptive agents, therapeutic ultrasonography, gene-editing strategies, and immunological approac...

Targeted allelic diversification of flowering genes via CRISPR-Cas enables the development of multiple early-flowering soybean lines

CRISPR-Cas multiplex editing generated a broad range of early-flowering soybean lines, advancing flowering by up to four weeks under controlled long-day conditions and by approximately one to two weeks in a field trial. Soybean is a promising crop for European agriculture, but its cultivation area remains smaller than...

Harnessing next-generation molecular technologies for precision control of Tuta absoluta and Helicoverpa armigera in tomato

Advanced molecular approaches providing sustainable resistance against insect pests have been discussed, like transgenic expression of insecticidal proteins, RNAi (RNA interference) strategies including HIGS and SIGS, genome editing by CRISPR-Cas, protease inhibitors and male sterility.

A ΦKMV ligase-dependent DNA repair mechanism that mitigates DNA-targeting nucleases

A broad-acting mechanism that allows Phikmvvirus phages to evade nuclease targeting is described and phage escape through faithful repair is described and a potentially interesting gene for phage therapy is identified.

Micronutrient Dynamics in Purple Cauliflower: Physiological Roles, Molecular Regulation, Anthocyanin Accumulation and Future Perspectives - A Critical Review

A comprehensive synthesis of micronutrient dynamics in purple cauliflower is provided and advanced biofortification models, nano-fertilizer integration, CRISPR/Cas gene editing, AI-assisted precision nutrition and multi-omics frameworks are critically evaluated to establish future strategies for climate-resilient and n...