gene editing

Dmp1 Knock-in Mice Faithfully Report and Manipulate Dmp1-Expressing Cells.

Two novel knock-in mouse lines using CRISPR-Cas9-mediated genome editing provide faithful tools for investigating the cellular and molecular mechanisms regulating dentinogenesis and osteocyte biology, as well as for studying dental and alveolar bone tissues, with potential applications in craniofacial biology.

Auto-SMART: a web-based platform for repair-template design for CRISPR/Cas9 homology-directed editing

Genome engineering via CRISPR/Cas9 has been widely applied in biological research and developing gene therapies. However, genome editing flexibility is strongly constrained by the position and quality of guide RNAs relative to the intended edit: desired edit sites are frequently positioned far from efficient protospace...

Inhibition of USP2 suppresses pathological pexophagy and rescues PEX1-deficient peroxisome biogenesis disorder

Clinically, PBDs are encompassed within the Zellweger spectrum disorders (ZSDs) and are encompassed within the Zellweger spectrum disorders (ZSDs) respectively.

Aspects of Precision Livestock Farming to Advance the Cattle Industry

Genetic engineering of the myostatin gene in dairy cattle represents a promising approach for sex-specific beef performance enhancement. A possible genetic strategy to enhance beef traits in male dairy calves uses the introduction of a myostatin knockout on the Y chromosome. Using CRISPR/Cas9, this sex-specific edit wo...

Editorial: Trends and perspectives for the use of crop wild relatives in crop breeding, volume II

Editorial on the Research TopicTrends and perspectives for the use of crop wild relatives in crop breeding, volume II Crop wild relatives (CWRs) are crucial assets for agrobiodiversity, sustainable agriculture, and food security, offering vital genetic traits such as pest resistance, resource efficiency, and extreme we...

CRYSPER and CUREIPES: two new molecular tools enabling analysis of RNA splicing and C-to-U RNA editing-inducible protein expression at single cell resolution

CUREIPES was developed and is the first molecular tool that enables human Apobec1-dependent C-to-U RNA editing-inducible protein expression, which can be used to counteract pathological alteration of C-to-U RNA editing through cell-autonomous expression of editing-regulatory proteins, or of any other protein of interes...

Microalgal proteins as sustainable alternatives: CRISPR opportunities and challenges.

This review systematically summarizes the comparative advantages, application prospects, and industrialization challenges of microalgal protein as an alternative protein source and provides an integrated framework for the low-cost, scalable, and sustainable production of microalgal alternative protein.

Molecular Mechanisms of WRKY Transcription Factors Integrating Environmental Signals to Regulate Flowering Time in Brassica Crops.

This review summarizes the structural, evolutionary, and functional characteristics of WRKY transcription factors involved in flowering regulation in Brassica species and demonstrates substantial expansion of WRKY gene families through genome triplication and polyploidization.

Characterization of Cellular Alterations in a Novel NAGLU Enzyme-Deficient Cellular Model Generated from U-87MG Cell Line

A glial cell model deficient in the NAGLU enzyme via CRISPR/Cas9 editing system in the U-87MG cell line exhibited typical traits for MPS IIIB, including decreased enzyme activity and mitochondrial mass, as well as increased lysosomal mass, neutral lipids, total glycosaminoglycans, and total reactive oxygen species.

Gene editing to improve stress resilience in wheat

Water deficit, salinity, and low-temperature stresses negatively affect wheat production worldwide, underscoring the need to develop stress-tolerant wheat varieties. This can be achieved by modifying the stress response mechanisms and regulations in plants. The recent DNA-editing approach, CRISPR/Cas editing, can accur...

Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells

CanProTarget

A residue-resolved prioritization platform for covalent cancer drug discovery. CanProTarget integrates cancer gene dependencies, small-molecule covalent ligand engagement measured by chemoproteomics, and base-editing evidence for individual cysteines, keeping each layer at the resolution it is measured at.