gene editing

An Overview of Programmable Epigenetic Editing Based on CRISPR Tools for Gene Expression Regulation.

An overview of the conceptual and technical foundations of CRISPR-based epigenetic editing, including tools for gene activation and repression, DNA methylation, histone modifications, and multiplexed systems that permit coordinated regulation of multiple genomic loci or epigenetic marks are provided.

Plant DNA Damage Response and Repair Pathways: from Molecular Mechanisms to Crop Improvement

This review synthesises the molecular basis of plant DNA damage responses and major repair pathways, with emphasis on their coordination and relevance to crop improvement.

Oligo-Modified Poly(β-Amino Ester)-Mediated mRNA Delivery: A Protocol for Gene Editing Applications.

A protocol for preparing OM-pBAE nanoparticles is described, including polymer synthesis, peptide modification, controlled mixing with nucleic acids to form polyplexes, precipitation in RNase-free water, stabilization with excipients, and optional freeze-drying for long-term storage.

Editorial: Stress tolerance in sorghum: molecular mechanisms, gene discovery, and quality dynamics

Climate change-driven abiotic stressesdrought, salinity, and waterloggingare the primary yield-limiting barriers for sorghum cultivation worldwide, and six papers in this collection dissect the morphological, physiological, and genetic regulatory networks governing sorghum stress tolerance. Drought is the most pervasiv...

Converging GWAS and Genome Editing Decrypts MsCBF8‐ MsGolS2 Regulatory Module for Drought Tolerance Without Yield Penalty in Medicago sativa

ABSTRACT Drought constitutes a major abiotic stress limiting alfalfa productivity. To elucidate the genetic basis of drought tolerance, a genome‐wide association study (GWAS) was conducted in alfalfa, which identified a CBF/DREB1 family transcription factor designated MsCBF8. Utilizing an optimized CRISPR‐Cas9 system,...

Guide reselection expands canonical PAM candidates in an HGD editing resource for alkaptonuria

Fine-Tuning Electroporation and AAV Delivery Parameters Balances Embryo Developmental Competence and Genome Editing Efficiency

Genetic and Molecular Basis of Processing Traits and Genetic Improvement Progress in Major Fruit Crops

Fruit processing plays a critical role in reducing postharvest losses, enhancing value addition, and improving the utilization efficiency of horticultural produce. However, most fruit breeding programs have historically prioritized fresh‐market traits such as yield, appearance, shelf life, and disease resistance, whe...

Phenotype-genotype association for the identification of resistance to stem borers (Diatraea spp., Lepidoptera: Crambidae) in sugarcane

These results provide a foundation for the identification of genetic resources for Diatraea spp.

ZmWGSL2 Is a Candidate Chloroplast-Localized PPR Gene Associated with Chloroplast Development and Leaf-Colour Formation in Maize

The characterized wgsl2-0, a stable white-green striped leaf mutant isolated from an ethyl methanesulfonate-mutagenized RP125 population of maize, provides strong genetic evidence for ZmWGSL2 as the most likely candidate gene associated with chloroplast development and leaf-colour formation in maize.

Gene editing technologies in poultry: advances, applications, and future prospects

SUMMARYThe chicken is an important model organism for research and a major global source of animal protein (eggs and meat). Recently developed genome-editing technologies have allowed the production of genetically engineered animals for agriculture and food production. Genome editing in chicken is a promising approach...

CRISPR/Cas9 Gene Editing of OsSD1 and OsDEP1 Improves Plant Architecture and Yield in Indigenous Black Rice Cultivar Chakhao Amubi

Rice ( Oryza sativa L.) sustains over half of the global population. The black rice landraces of North-East India are agronomically limited by tall stature, lodging, and low yield. We employed CRISPR/Cas9 -mediated multiplex genome editing to improve the elite aromatic black rice cultivar Chakhao Amubi by targeting thr...