gene editing

Chromosome Editing Using the Lambda Red Recombinase System in Chlamydia trachomatis and Chlamydia muridarum.

Genome edited sheep generated by zygote electroporation of prime editor ribonucleoproteins

Prime editing (PE) enables precise genome modifications without double-strand breaks, yet its application in large mammals has been hindered by low efficiency and the technical bottlenecks of zygote microinjection. This study aimed to establish a scalable PE platform in sheep via the direct electroporation of a PE3 RNP...

Genome editing frontiers for developing abiotic stress tolerant and climate resilient crops

Gene editing applications, opportunities and challenges for crop improvement in Africa a review

Gene editing, particularly CRISPR/Cas-based new genomic techniques (NGTs), offers significant opportunities for accelerated crop improvement in developing African countries, ensuring food security, livelihoods, climate resilience, and economic growth. This review critically evaluates the scientific basis, applications,...

Hypertrophic Wasp Stem Cell Bio-Hybrid Muscle Actuator Powered and Cryogenically Cooled by Ethanol Solution

A novel theoretical concept in bio-hybrid robotics and tissue engineering: a macroscopic biological muscle actuator engineered from the stem cells of the Oriental Hornet (Vespa orientalis). The system utilizes CRISPR-Cas9 genome editing to multiply the Alcohol Dehydrogenase (NADP+) gene for absolute alcohol immunity, c...

Language-Model-Based Detection of Genetic Editing in Bacteria

A transformer-encoder-based machine-learning classifier that, instead of analyzing words in sentences, models gene families in genomes, provides a scalable method for identifying engineered sequences without relying on specific marker genes, with potential applications in biosecurity, agriculture, GMO regulation and mo...

Measurement reliability bounds functional benchmarks and relocates where variant effect prediction fails

Background Variant effect predictors are increasingly benchmarked against multiplexed assays of variant effect (MAVEs) rather than clinical labels, which removes label circularity but introduces a new problem: a correlation against a measurement cannot exceed the measurement’s own reproducibility, and precision varies...

Multiscale transcriptomic analysis separates epithelial identity loss from FN1-associated stromal remodeling in ulcerative colitis: reproducibility package

This repository contains the reproducibility materials supporting the manuscript “Multiscale transcriptomic analysis separates epithelial identity loss from FN1-associated stromal remodeling in ulcerative colitis.” The archive includes analysis and figure-generation scripts, frozen and processed source tables used for...

Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice

Drought poses a major threat to rice ( Oryza sativa ) production, causing severe yield losses and jeopardizing yield stability. C2H2 Jumonji domain–containing zinc-finger proteins play diverse roles in plant growth, development, and abiotic-stress responses through regulation of gene expression and chromatin-associated...

Multiscale transcriptomic analysis separates epithelial identity loss from FN1-associated stromal remodeling in ulcerative colitis: reproducibility package

This repository contains the reproducibility materials supporting the manuscript “Multiscale transcriptomic analysis separates epithelial identity loss from FN1-associated stromal remodeling in ulcerative colitis.” The archive includes analysis and figure-generation scripts, frozen and processed source tables used for...

Advancing the sterile insect technique for the New World screwworm fly: the case for CRISPR-engineered genetic sexing strains.

It is argued that establishing a screwworm GSS would provide immediate benefits to existing management programs and hope this synthesis will inform and encourage future studies on this effort.

Multiplex CRISPR/Cas12a editing of ROC5, GS3, GW2, and LARGE2 reveals effects on yield-related traits in rice cultivar Kasalath