protein folding

Tree-aware conditional language modeling recovers mutational patterns of viral evolution

EvoPLM-Tree, a tree-aware conditional autoregressive language model that predicts descendant protein sequences from ancestral sequences together with phylogenetically derived evolutionary features, provides a framework for modeling protein evolution along phylogenetic lineages and prioritizing plausible future mutations from genomic surveillance data.

Predictive all-atom simulations of disordered proteins and biomolecular condensates through osmometry-guided force-field optimization

Multi-knockout of 29 phytotoxic proteins and metabolites does not completely abolish virulence of the necrotrophic fungus Botrytis cinerea

This work demonstrates that necrotrophic pathogenesis in B. cinerea does not depend on a few primary virulence determinants, but rather on a highly redundant network of host damaging factors.

Novel Eukaryotic Double Histone Fold Motifs Sharing High Sequence Identity with Histones H3 and H4 in Single-Domain Proteins and in Proteins Featuring Remarkable Histone Fold Multiplets

A large number of DHF proteins featuring a high or very high degree of identity with the amino acid sequences of both histones H3 and H4 are identified, which constitute a new class of eukaryotic DHF proteins.

PRDX1 drives colorectal cancer progression and immune microenvironment remodeling by facilitating PRMT5 nuclear translocation to activate Wnt/β-catenin signaling.

Findings identify a novel PRDX1-PRMT5 axis that activates Wnt/β-catenin signaling, highlighting a potential therapeutic strategy for CRC by targeting this pathway to suppress tumor progression and remodel the immune microenvironment.

Structural basis of Saccharomyces cerevisiae Mba1 in mitochondrial co-translational membrane insertion.

The solution structure of mature Saccharomyces cerevisiae Mba1 is determined using multidimensional nuclear magnetic resonance (NMR) spectroscopy and reveals a compact α + β fold with a central hydrophobic cavity and distinct charged surface regions, providing a structural framework for interpreting previous functional studies of Mba1.

Structural and energetic landscape of the human HOP-PrPC Axis: Multi-domain stabilization and peptide-mediated disruption by melittin.

These findings demonstrate that the conformational plasticity of HOP can be strategically exploited for targeted protein-protein interaction (PPI) disruption in cancer therapy.

Characterization of hemp seed oil-carnauba wax oleogel and the effect of oleogel usage and hemp seed flour substitution on the properties of wheat flour crackers.

This study investigated the potential use of hemp seed oil-carnauba wax oleogel as a margarine substitute in crackers and the effects of substituting wheat flour with hemp seed flour. Characterization revealed that 11% wax oleogel best mimicked margarine. Control crackers contained margarine (C1) and oleogel (C2). Wheat flour in C2 was substituted with 10% (HSF10) and 20% (HSF20) hemp seed flour. Oleogel increased unsaturated fatty acids by 76.84%. Although oleogel restricted the spread ratio, hemp seed flour increased it. HSF20 enhanced the protein, ash, and fat by approximately 1.4-fold and fiber by 5.68-fold compared to C1. Hemp seed flour reduced fat migration and increased the phenolics and antioxidant activity by >2-fold, controlling lipid oxidation. Sensory analysis showed no significant differences in taste/odor among samples, but C2, HSF10, and HSF20 received higher scores for chewiness. These findings highlight improvements in the crackers' nutritional, oxidative, and bioactive properties without compromising sensory taste.

The fibrocystin C-terminal domain inhibits Src/STAT3 signal induced cystogenesis of kidney epithelial cells.

FPCct appears to act like a physiological suppressor of cystogenic signaling, as found in healthy kidney epithelia, that is essential for maintaining epithelial homeostasis.

Structural insights into Cas9 inhibition by AcrIIA17 via bridge helix interaction

The crystal structure of AcrIIA17 is presented and its mechanism of Staphylococcus aureus Cas9 (SauCas9) inhibition is elucidated, identifying AcrIIA17 as an Acr protein that targets the Cas9 BH domain and reveal the BH domain as a regulatory checkpoint in Cas9 activation.

Exploratory Analysis of Type 2 Diabetes Mellitus Metabolic Phenotypes and Their Association With Vitamin D Status in Primary Care Patients.

The metabolic phenotypes were explored in a primary care T2DM cohort, supporting their reproducibility across clinical settings and vitamin D status varied by phenotype severity, with more severe phenotypes more likely to exhibit insufficiency.

The Blumeria graminis Effector BgtH12 Suppresses Wheat Immunity and Acts as a Virulence Factor.

BgtH12 is established as a critical virulence factor that suppresses basal immunity to promote Bgt colonization and stable overexpression of BgtH12 in wheat enhanced susceptibility.