TL;DR
The adaption of the continuous directed evolution (CDE) along with base editor (BE) gene editing strategy to discover TEM-1 mutants is reported here and highlights the application of gene editing methods to provide insight into the molecular basis of antibiotic resistance.
It is discovered that the mere presence of effector genes of resistance, as well as conserved gene regulatory architecture, do not necessarily translate to similar evolvabilities during antibiotic challenge, and the importance of understanding pathogen-specific genotype-phenotype maps of drug resistance to better predi...
Core genome variation is highlighted as a potential contributor to antimicrobial resistance in K. pneumoniae and conserved mutations such as metG Asn366Asp may represent candidate genomic biomarkers warranting further investigation, which are regarded as hypotheses for future experimental testing.
ABSTRACT Antibiotic resistance is a growing challenge in the treatment of bacterial infections, prompting interest in alternative therapies. Understanding how bacterial populations evolve under different selective pressures is important for predicting resistance pathways. This study aimed to investigate the evolutionar...
The continuous evolutionary race between antibacterial innovation and antimicrobial resistance (AMR) has become one of the greatest challenges in the management of Gram-negative bacterial infections. Although numerous antibiotics and β-lact- am–β-lactamase inhibitor combinations have been introduced since 2000, their c...
Conjugation experiments demonstrated that the β-lactam resistance gene blaOXA-246 could be horizontally transferred from the donor MD9 strain to the recipient Escherichia coli 25DN strain, thereby potentially contributing to the dissemination of multidrug-resistant bacteria.
An IS26-driven circular blaKPC-2 transposon is reported in ST2483 P. aeruginosa, conferring robust overexpression and stable inheritance in the donor strain, with OMVs potentially facilitating its horizontal spread, although stable maintenance in recipient cells was not demonstrated under the current assay conditions.
General Science
A Blog post by NVIDIA on Hugging Face
Zero-knowledge proofs can verify computing results without rerunning workloads or exposing sensitive data. Magnetar helps bring them into the mainstream with support for standard programming languages, major speed gains, and quantum-resistant security. The post Proof-based general-purpose computing with Magnetar appeared first on Microsoft Research.
General Science
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