High-level expression and characterization of a thermostable thermolysin-like metalloprotease with industrial potential.

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

The biochemical characterization and crystal structure of Ta0887, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum, provide a basis for the future engineering of Ta0887 with the aim of enhancing its potential for industrial and biotechnological applications.

Identification of a Novel Thermal Promoter and Its Application in Glutamate Decarboxylase Protein Expression in Bacillus licheniformis.

The identified novel temperature-responsive promoter, PycgM, is a robust, high-temperature genetic element for efficient enzyme and metabolite production in thermotolerant hosts, demonstrating excellent compatibility with a thermotolerant host.

[Rational design for improving the thermostability of β-glucosidase from Bacillus thermoamylovorans].

A Ca2⁺-responsive GH37 neutral trehalase from Lentinula edodes: Cloning, heterologous expression and biochemical characterization.

Trehalases (EC 3.2.1.28) hydrolyze trehalose into two glucose molecules and play important roles in fungal trehalose metabolism. Although GH37 neutral trehalases have been characterized in yeasts and filamentous fungi, their biochemical properties in basidiomycetes remain poorly understood. In this study, we cloned LeN...

Genomic prospecting and biochemical characterization of a novel thermostable 3-quinuclidinone reductase from hot spring metagenomes for efficient biocatalysis

The discovery and characterization of a novel thermophilic 3-quinuclidinone reductase identified through metagenomic mining of hot spring environments establishes ScQR as a robust biocatalyst for high-temperature pharmaceutical applications and demonstrates a scalable workflow for optimizing enzymes from extreme enviro...

An integrated engineering strategy enhances expression and activity of Pseudonocardia thermophila nitrile hydratase in Escherichia coli.

Nitrile hydratase is a key enzyme for nitrile-to-amide hydration under mild conditions, yet its application is limited by low heterologous expression, poor solubility, and suboptimal catalysis. Here, we present an integrated strategy to enhance expression, assembly, and function of Pseudonocardia thermophila NHase (PtN...