Skip to content
Log in
Sponsored AI AM CLAUDE, A Beginner's Guide: Learn the basics of working with AI AI AM CLAUDE, A Beginner's Guide: Learn the basics of working with AI
Open access

Germline-encoded V(D)J gene usage does not impose strict constraints on the epitope-specificity of T cell receptors

Adrian Straub Yang An F. Drost Kersten Heyer Zahra Abedi M. Hammel Abagail M. Delahoussaye Sabrina Wagner Anton Mühlbauer Julian Hönninger Jack Barton Karl Moukarzel L. Warmuth Sarah Braun L. Valentiner Corinne Angerpointner Thomas Pohl V. Buchholz Kilian Schober M. Warkotsch Benjamin Schubert Dirk H. Busch
Aug 2026 · bioRxiv · 0 citations · 67 references
Biology

TL;DR

Diversity of epitope-specific TCRs is not limited by distinct germline combinations, but rather can emerge from a near-to-complete combinatorial space of α- and β-chain, V and J segments paired with compatible CDR3 sequences.

DOI Semantic Scholar
Read PDF

Similar papers

Open access Sep 2026

MHC restriction is structurally encoded by the T cell receptor germline

MHC restriction — the requirement for αβ T cell receptors (TCRs) to recognize Major Histocompatibility Complex (MHC) molecules when presenting peptide antigens — is a pillar of adaptive immunity. Here, we provide direct structural evidence for TCR germline-encoded recognition of MHC molecules irrespective of antigenic...

Nan Wang, Marta T. Borowska, K. Jude et al. · 0 citations
Open access Aug 2026

Functional Pairing of TCR-peptide-MHC Interactomes by Single-cell Clonal Expansion

The LINC-seq platform, LINC-seq, recovered known cognate pairs from pooled libraries at up to 95% accuracy and performed simultaneous, library-on-library deep mutational scanning of both partners, and generalizes to any receptor-ligand pair whose trans-engagement drives a reporter.

L. Liu, Seung Won Shin, Kevin M. Joslin et al. · 0 citations
Review Open access Oct 2026

Entering the Age of Specificity in T Cell Immunity

ABSTRACT The discovery of MHC restriction, the resolution of peptide–MHC structure, and the identification of the T cell receptor gene quickly centered epitope‐level specificity as a core concern of T cell biology. Epitope mapping culminated in functional peptide‐based stimulation assays and tetramer staining, which to...

Paul G. Thomas · 0 citations
Open access Sep 2026

Structure-function profiling identifies determinants of TCR-T cell therapeutic efficacy

A mechanistic basis and a conceptual framework for the selection and optimization of TCRs for adoptive immunotherapy are provided for the selection and optimization of TCRs for adoptive immunotherapy.

Leire Oyon-Olea, Enric Vercher, M. G. Dichiara-Rodríguez et al. · 0 citations
Open access Oct 2026

SEEKER: A genome-scale library-on-library screening platform for deciphering T cell recognition of antigen

Decoding which antigens activate a given T cell receptor (TCR) is a central challenge in immunology. We present SEEKER, a functional genome-scale library-on-library screening platform built on three elements: a Jurkat-derived T-APC cell (TAPCell) that combinatorially expresses one TCR and one peptide–MHC drawn from sep...

Si-Cheng Li, Li Lin, Wen-Xu Wu et al. · 0 citations
Open access Sep 2026

Unveiling structural determinants of peptide recognition by public and private SARS-CoV-2-specific T-cell receptors

Public T cell receptors (TCRs) recurrently emerge across individuals in response to common pathogens, yet the structural and biophysical basis distinguishing public from private clonotypes remains incompletely defined. Here, we combine epitope mapping, single-cell TCR sequencing, and single-particle cryo-electron m...

Caner Akıl, Yan-Chun Peng, Julia McCarthy et al. · 0 citations

We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.