Fiber-seq exposed widespread plasticity in the linear organization of individual chromatin fibers and illuminated principles guiding regulatory DNA actuation, the coordinated actuation of neighboring regulatory elements, single-molecule nucleosome positioning, and single-molecule transcription factor occupancy.
Chromatin accessibility has long been used as a marker for regions of DNA with regulatory potential. Fiber-seq detects chromatin accessibility on individual DNA fibers, enabling analyses beyond the identification of the accessible chromatin regions (ACRs). By providing single molecule level high resolution, Fiber-seq p...
K. Bubb, Molly Perchlik, J. Cuperus et al.· bioRxiv· 0 citations
The six-residue loop 2 of H2A and H2A is used to map, at single-residue resolution, how nucleosome-core variation reshapes cellular function, and identifies histone variation as a source of phenotypic innovation.
Zachary H. Harvey, Benjamin Gundinger, Jian-Yi Kok et al.· bioRxiv· 0 citations
Nucleosome positioning plays a fundamental role in regulating DNA accessibility and cellular activity, yet the precise rules by which mammalian sequences instruct this organization have remained incomplete. Prior work has established that intrinsic sequence preferences dictate nucleosome architecture in yeast, but in m...
M. Christophe, L. Altamirano, P. Navarro et al.· bioRxiv· 0 citations
A detailed protocol for flap-enabled next-generation capture (FENGC), a cost-effective method for targeted, multiplexed enrichment of DNA sequences for epigenetic and genetic analysis.
Mingqi Zhou, Marie-Pierre L. Gauthier, N. Nabilsi et al.· Methods in molecular biology· 0 citations
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