Analytical methodology for unveiling the physical effect of detergents on the function of full-length membrane proteins using a single-molecule tracking system
Clear trade-offs are revealed between detergents that excel in membrane solubilization and those that better preserve conformational stability, with no single detergent outperforming across all metrics.
Sibel Kalyoncu Uzunlar· Cumhuriyet Science Journal· 0 citations
A generalizable workflow for producing soluble receptor domains from membrane proteins is established and validated HasR constructs suitable for downstream ligand-screening applications, including aptamer and nanobody discovery.
Federico Bosetto, Maria Zacharopoulou, M. Bycroft et al.· bioRxiv· 0 citations
The protocol for carrying out sedimentation velocity-type analytical ultracentrifugation experiments is presented and developed to help understand the mechanism of multi-component tripartite ATP-independent (TRAP) transporter proteins, which are essential for nutrient uptake in bacteria.
Zachary D. Tillett, R. Dobson, R. A. North· Methods in molecular biology· 0 citations
Together, the methods described here demonstrate that careful selection via thorough screening, and optimization of expression and purification conditions, are an invaluable investment of time, ultimately yielding the best target not only for successful high-resolution structural analyses-X-ray crystallography and cryo...
B. Kloss, C. Ardiccioni, V. Petrou et al.· Methods in molecular biology· 0 citations
G protein-coupled receptors (GPCRs) are membrane proteins that act as signal transducers across cell membranes. Class B1 GPCRs, a subset of 15 receptors activated by peptide hormones, are involved in important physiological processes and diseases, making them a popular target for drug development. GPCRs are dynamic pro...
Theodore J. Nettleton, P. M. Sexton, D. Wootten et al.· Biochemical Society Transact...· 0 citations
G protein-coupled receptors (GPCRs) represent the largest membrane protein family in the human body and the major targets of FDA-approved medications. Unlike some other physiological proteins, GPCRs are highly plastic and often exist as collections of conformational ensembles in the environment to exert their function....