Accurate chromosome segregation relies on kinetochores maintaining load-bearing attachments to dynamic microtubule ends, a coupling in which the Dam1 complex ring is central. How the microtubule-end structure and ring–microtubule interactions collectively determine attachment stability and drive force transduction rema...
Maksim A. Kalutskii, H. Grubmüller, M. Igaev· bioRxiv· 0 citations
During eukaryotic cell division, kinetochores couple duplicated sister chromatids to mitotic spindle microtubules to mediate faithful chromosome segregation. Although the main kinetochore attachment sites to centromeric chromatin and microtubules are known, additional factors including microtubule-associated proteins a...
Yvonne B. Winterborn, Christopher Batters, Tomos Morgan et al.· bioRxiv· 0 citations
Centromeres, defined by CENP-A–containing nucleosomes, direct kinetochore assembly for spindle attachment. In mitosis, CENP-A and the constitutive centromere-associated network (CCAN) of the inner kinetochore are arranged into bipartite subdomains within clearings of chromatin. However, less is known about their archit...
It is shown that Naegleria kinetochores associate with chromatin and spindle microtubules and that their number is consistent with one kinetochore per chromosome, consistent with a model in which the reliance on bulk charge complementarity rather than residue-specific interactions permits significant sequence divergenc...
Andrew S. Kennard, Emily M. Larkin, Jacob M. Ritz et al.· bioRxiv· 0 citations
How the metaphase spindle in higher eukaryotes maintains stability in the presence of poleward microtubule (MT) flux is a confusing issue. Here, we present a model for the spindle by incorporating augmin-mediated MT connections and MT crosslinking by NuMA proteins. On the basis of the model, we study computationally...
Yao Wang, Yu-Ru Liu, Peng-Ye Wang et al.· Journal of Physical Chemistr...· 0 citations
Kinetochores are multiprotein complexes that drive chromosome segregation in eukaryotes. Kinetoplastids, a group of early-diverging eukaryotes that lack canonical kinetochore components, have a unique set of kinetochore proteins. How their kinetochores are assembled specifically at centromeres in the absence of a centr...
Aleksandra Ciszek, Patryk Ludzia, G. Marcianò et al.· bioRxiv· 0 citations
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