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CRISPR RNA-independent activation of Cas12a

I. Iwe S. Singh K. Guan R. F. Ocampo S. J. Ribeiro da Silva D. Wachholz Junior N. Emami A. Corsano I. Zeisler K. Bozovicar L. Wang D. Ham R. Cai P. Kelly R. Zayeni J. Nguyen P. Bayat M. Charania S. Palter F. X. Liu S. Shrestha A. Rayhan G. Wasney T. Mazzulli A. A. Green Z. Li S. Yao B. P. Hubbard D. Taylor K. Pardee

Jul 2026 · medRxiv · 0 citations

Medicine

TL;DR

It is shown that short RNAs can directly occupy the canonical crRNA-binding channel and trigger a catalytically competent trans cleavage state in the absence of PAM recognition or canonical R-loop formation.

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