Aug 2026· Protein engineering, design & selection : PEDS· Vol 39· 0 citations
Medicine
TL;DR
Findings indicate that phage propagation in Omptin-expressing bacteria leads to the loss of dibasic amino acid motifs from phage-displayed combinatorial peptide, protein, and antibody libraries, which suggests that the presence of dibasic residues in proteins and peptides displayed on bacteriophage M13 should necessitate the use of OmpT- and OmpP-deficient bacterial hosts.
Proteases responsible for leader peptide removal are ubiquitous in ribosomally synthesized and post-translationally modified peptide (RiPP) biosynthetic pathways. However, many characterized RiPP proteases require specific core peptide modifications prior to cleavage, limiting their broader applicability. Here, we id...
Yu-Kun Zhou, Guang-Zhao Xiao, Si-Cong Yao et al.· Journal of the American Chem...· 0 citations
The findings suggest that the YabG substrates include the basement layer and inner coat proteins and YabG processes these substrates after localizing to the basement layer and inner coat.
Ritsuko Kuwana, Y. Kasahara, K. Ito et al.· Frontiers in Microbiology· 0 citations
Abstract Membrane‐embedded bacterial receptors are challenging to express and purify in soluble form, yet their isolated domains are essential tools for structural and ligand‐discovery studies. Pseudomonas aeruginosa relies on the TonB‐dependent heme receptor HasR for iron acquisition, a process central to its pathogen...
Federico Bosetto, M. Zacharopoulou, M. Bycroft et al.· Protein Science· 0 citations
Sortase A (SrtA) enzymes covalently anchor surface proteins to Gram-positive bacterial cell walls, promoting colonization and virulence. In Streptococcus pneumoniae, previous studies identified both a domain-swapped dimer and an active refolded monomer, but the active enzyme has not been characterized at the structural...
Eunjeong Lee, Blaine H. Gordon, J. Redzic et al.· Biomolecules· 0 citations
The 3.0 Å cryo-electron microscopy apo-structure of the PGT enzyme WbaP from Escherichia coli, a UDP-galactose:undecaprenolphosphate galactose-1-phosphoryl transferase, provides new insights into the PGT family of enzymes.
M. Weckener, Audrey Le Bas, Philip N. Ward et al.· Open Biology· 0 citations
The soluble expression of EKL in E. coli is achieved and a highly active mutant is obtained, and a visual tool for the rapid detection of EKL activity is provided.
Chang-Chun Han, Jun-Hao Yue, Yuwen Tu et al.· Sheng wu gong cheng xue bao...· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
