Use of tRNA-Mediated Suppression to Assess RNA Chaperone Function.

Analysis of tRNA Aminoacylation by Northern Blotting and Multiplex Small RNA-Seq.

Quantification of tRNA aminoacylation has been used to demonstrate RNA chaperone activity for RNA-binding proteins that promote tRNA folding to increase the fraction of aminoacylated tRNAs, underscoring the utility of this assay in interrogating RNA chaperone-assisted tRNA folding and function.

Salt-Dependent Modulation of the RNA Chaperone Activity of RNA-Binding Protein La.

m1A58 acts as a conformational checkpoint coupling human initiator tRNA maturation to translation initiation

Findings establish m1A58 as a conformational checkpoint coupling human initiator-tRNA maturation to translation initiation and stress responses, and establish m1A58 as a conformational checkpoint coupling human initiator-tRNA maturation to translation initiation and stress responses.

Mapping the RNA Chaperone Activity of the Trypanosoma brucei Editosome Using SHAPE-Chemical Probing.

16S ribosomal RNA modification drives transcript-specific translation efficiency

It is proposed that MraW modification of 16S rRNA enhances translation efficiency in general, and that specific transcripts have evolved structural features that fine-tune protein levels that may be prevalent in bacteria which exhibit uncoupled transcription and translation.

Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding.

A general method for measuring energy landscape transformation that yields key physical insights into multiple functions of hairpin-binding proteins is illustrated.