The interaction between PolI with the β-clamp, mediated by a CBM unique to the Mycobacteriales order, highlights the mechanistic distinctiveness of genome replication in Mtb and phylogenetically related organisms, and has important implications for drug discovery.
In bacteria, DnaB replicative helicases are essential for unwinding genomic DNA to provide the single‐stranded templates required for replication. The recruitment of the hexameric DnaB helicase to the origin of replication is mediated by distinct mechanisms that rely on specialized factors known as helicase loaders. Th...
Daniele Mazzoletti, Andrea Garavaglia, Hayden Fisher et al.· Protein Science· 0 citations
The process of DNA transcription leads to the generation of torsional stress, which must be resolved for smooth progression of the transcription machinery. In Saccharomyces cerevisiae, DNA topoisomerase I (Top I), a type IB topoisomerase, plays a critical role in relaxing supercoils and mitigating the topological s...
Prakash Nandi, I. M. Kamal, Saikat Chakrabarti et al.· Frontiers in Bioinformatics· 0 citations
The transcription factor IdeR regulates iron homeostasis in many gram-positive bacteria. It primarily functions as a repressor of iron uptake genes by binding to their promoters and blocking RNA polymerase access. IdeR recognizes a highly conserved DNA sequence consisting of four palindromic 5’-C1C2T3A4A5 repeats. For...
Suvankar Ghosh, O. Koshla, D. Maurer et al.· bioRxiv· 0 citations
The results reveal a new C-terminus-mediated RPA-RecQ interaction in L. donovani that resembles the prokaryotic SSB-RecQ interaction mechanism, while maintaining functional conservation of eukaryotic DNA metabolism.
Mayukh Chakraborty, Susmita Ghosh, Surasree Chakraborty et al.· The FEBS Journal· 0 citations
These findings reveal remarkable architectural flexibility, mechanistic robustness, and a conserved mechanism in oriC, and highlight unwinding stability as a key determinant of oriC adaptation to rapid cell growth, providing insight into the evolution of oriC architecture.
Ryusei Yoshida, Kazutoshi Kasho, S. Ozaki et al.· Nucleic Acids Research· 0 citations
Abstract Prokaryotic Argonautes (pAgos) are widely distributed and provide immunity against invading DNA. Based on their domain architecture, pAgos are classified into three major groups: long-A, long-B, and short pAgos. Among them, long-B pAgos remain the least understood subgroup. Here, we show that a long-B pAgo-nuc...
Min Liu, Qin Yu, Xiao-Ruo Zhang et al.· Nucleic Acids Research· 0 citations
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