Characterization of tri-culture alveolar-like organoids to study fibrotic lung diseases

Journal Pre-proof Bi-directional epithelial-mesenchymal crosstalk provides self-sustaining pro-fibrotic signals in pulmonary fibrosis

Mesenchymal Stem Cell Secretome Mitigates Inflammatory Endothelial Dysfunction.

BACKGROUND Endothelial cells (EC) dysfunction and barrier disruption are central drivers of inflammatory lung injury and adverse outcomes, yet targeted therapies remain lacking. We tested the hypothesis that a mesenchymal stem cell (MSC)-derived secretome can directly preserve EC function under inflammatory stress. We...

An hiPSC-derived multi-lineage lung model exhibiting proximal-distal epithelial features for modeling pulmonary fibrosis

A humanized multi-lineage lung model that captures epithelial-mesenchymal co-development and responsiveness to TGF-β1 stimulation and sensitivity to pirfenidone highlight its potential for antifibrotic drug screening.

Optimization of a Canine Colon Organoid Platform for Modeling Intestinal Injury and Mesenchymal Stem Cell-Mediated Regeneration.

Canine chronic enteropathy (CE) is characterized by persistent mucosal inflammation and impaired barrier function. Despite its high clinical prevalence, research has been hindered by a lack of host-specific intestinal models. Here, we present an optimized canine-derived colon organoid (cCO) culture platform designed fo...

Therapeutic Effects of Wnt5a-Overexpressing Bone Marrow Mesenchymal Stem Cells on Endothelial Barrier Repair in Acute Lung Injury

Background: Acute lung injury/acute respiratory distress syndrome (ALI/ARDS), a common complication of sepsis, is critically characterized by disruption of the alveolar–capillary barrier. The use of mesenchymal stem cells (MSCs) has emerged as a promising therapeutic strategy for ARDS owing to their potent paracrine ef...

The role and mechanism of macrophage-to-myofibroblast transition in fibrotic diseases

Fibrosis is a pathological process primarily characterized by excessive deposition of extracellular matrix (ECM), which leads to disruption of tissue architecture and progressive loss of organ function, serving as a key pathological basis for various chronic diseases. Recent studies have revealed that, in addition to c...