Open access

An in vitro System for Studying Osteochondrogenic Differentiation of Smooth Muscle Cells and Modeling Intimal Vascular Calcification

J. Monteiro Matthew D. Worssam Wen-Duo Gu D. Li Quan-Yi Zhao A. K. Karius M. Ramste G. Qin I. Damiani Si-Wen Zheng Trieu D. Nguyen Brian T. Palmisano Chad S. Weldy J. Kim M. Kaikkonen P. Cheng T. Quertermous

Sep 2026 · bioRxiv · 0 citations · 39 references

Medicine Biology

TL;DR

The osteochondrogenic differentiation assay results in a strong calcification phenotype together with a disease-associated CMC-like transcriptional state, providing a reliable in vitro model for mechanistic investigation of SMC phenotypic transition and prioritization of candidate regulators.

DOI PubMed Semantic Scholar

Read PDF

Similar papers

Open access Aug 2026

Single-cell RNA sequencing reveals chondrocyte heterogeneity and fibrocartilage transition in osteonecrosis of the femoral head

Summary Cartilage degeneration in osteonecrosis of the femoral head (ONFH) is closely associated with chondrocyte heterogeneity. Single-cell RNA sequencing was performed on 50,851 chondrocytes from 10 ONFH patients and 10 controls. The findings were further validated using an in vitro chondrocyte injury model induced b...

Tun Liu, Kai Nan, Xiao-Qian Dang et al. · iScience · 0 citations

Sep 2026

The osteogenic regulator THOC5-dependent post-transcriptional control maintains vascular smooth muscle cell homeostasis against chronic kidney disease related vascular calcification.

INTRODUCTION Osteogenic transdifferentiation of vascular smooth muscle cells (VSMCs) plays a fundamental role in chronic kidney disease (CKD)-associated vascular calcification (VC). Despite its significance, the post-transcriptional control mechanisms driving VSMC osteogenic switching remain poorly understood. Here, we...

An-Ying Cheng, Yu-Yao Wang, Yu Liu et al. · Kidney International · 0 citations

Open access Aug 2026

An ncBAF-ETS2 Chromatin-Remodelling Axis Drives Vascular Smooth Muscle Cell Osteogenic Reprogramming in Vascular Calcification

Introduction Vascular calcification is a detrimental ageing-related pathology that is markedly accelerated in metabolic disorders. It is driven by osteogenic differentiation of vascular smooth muscle cells (VSMCs), however epigenetic regulatory pathways activated early in this transition remain poorly defined. Methods...

Meng-Ying Wu, Jirapath Thammaphet, Audrey Kelly et al. · bioRxiv · 0 citations

Open access Sep 2026

Dermal bone remodelling by invasive osteoblasts evolved in stem gnathostomes

The evolutionary origin of the human face can be traced to the earliest vertebrates with a dermal skeleton in which canonical skeletal tissues and cell types arose. To elucidate its developmental evolution we performed genetic mosaic labelling, 3D single cell analysis and fluorescent matrix birthdating in vivo. We disc...

K. Jordan, Jason P. Downs, Xin-Tao Zhang et al. · bioRxiv · 0 citations

Open access Sep 2026

Chaenomeles speciosa-derived extracellular vesicles accelerate fracture healing via orchestrating immuno-angiogenic coupling

Purpose Fracture nonunion remains a major orthopaedic challenge, and dysregulation of the bone immune microenvironment is increasingly recognized as a key pathological driver. Chaenomeles speciosa is a medicinal plant with documented anti-inflammatory properties, making it a promising candidate for immune-modulatory th...

Jing-Xiang Chen, Kai-Han Zhuang, Yufei Wang et al. · Bone Reports · 0 citations

Open access Aug 2026

Inhibition of EED enhances osteogenic differentiation and bone formation: a potential therapeutic strategy for osteogenesis imperfecta

It is demonstrated that inhibition of the PRC2 through targeting its essential component embryonic ectoderm development (EED) enhances osteogenic differentiation, improves bone architecture in male Col1a2+/G610C OI mouse models, modulates the integrated stress response (ISR), and improves ER morphology in OI cells.

Margarita E. Carrasco Jeldres, Oksana Pichurin, S. Weaver et al. · JBMR Plus · 0 citations