Jun 2026· International Journal of Current Pharmaceutical Review and Research· Vol 18· 0 citations
Abstract
Background: The genus Burkholderia, which was previously believed to be a soil saprophyte, is now gaining attention as a human disease. Pathogenic species include B. pseudomallei, B. cepacia, B. mallei, B. gladioli, and B. thailandensis. Ongoing studies on Bukholderia have led to the isolation of more recent subspecies from human samples. The purpose of this study is to assess the antibiogram of the clinical isolates and determine the prevalence of Burkholderia spp. Methods: Demographic data and a variety of patient clinical samples were analyzed. All specimens were processed using recognized microbiological techniques. Results: Of the 8230 culture-positive samples, 1902 (23.11%) included Non-Fermenting Gram Negative Bacilli (NFGNB). It was discovered that 60 (3.2%) of these NFGNB were Burkholderia spp. Furthermore, it was found that 24 (40%) and 36 (60%) were B. pseudomallei and B. cepacia complex, respectively. Of the patients, 76.7% were over 40, and 80% were men. Diabetes mellitus was the primary risk factor (60%), and fever was the most common manifestation (53.3%). Imipenam had the lowest sensitivity, while Minocycline and Cotrimoxazole had the highest, according to antibiotic sensitivity testing. Conclusion: This study provides baseline data on the present Burkholderia infection situation at our hospital. An ongoing investigation will be useful in identifying several cases because this organism is extremely underreported.
The increase in antibiotic resistance observed for most of the antibiotics evaluated represents a relevant clinical challenge requiring continuous epidemiological surveillance and is a relevant clinical challenge requiring continuous epidemiological surveillance.
Vera Lucía Velásquez Ordóñez· Revista Científica del Sist...· 0 citations
Acinetobacter baumannii is a common multidrug-resistant bacteria that causes hospital-acquired illnesses. This research attempted to identify quinolone resistance genes in A. baumannii isolated from various clinical materials, including burns, blood, sputum, urine, and diabetic foot infections, with a distribution of 199 (53%) male and 176 (47%) female subjects. The bacterial culture results indicated that out of 375 specimens, 157 (41.9%) exhibited single growth, 28 (7.4%) displayed mixed growth, and 190 (50.7%) showed no bacterial growth. This study identified 155 instances (41.33%) of gram-negative bacteria. The Vitek-2 system identified 28 isolates (7.46%) as A. baumannii.
The highest isolation rate occurred in burn patients at 17 (4.53%), followed by 4 (1.07%) isolates from patients with respiratory tract infections, and 3 (0.8%) from blood source records. Diabetic foot infections and urinary tract infections were documented in two instances (0.53%). The antimicrobial susceptibility test results revealed that all 28 (100%) isolates demonstrated resistance to Cefoxitine, Ceftazidime, and Ciprofloxacin. Additionally, 27 (96.42%) of the isolates were resistant to meropenem, while 26 (92.85%) exhibited resistance to tobramycin and Ampicillin-Sulbactam. Concurrently, 89.28% of the isolates were resistant to gentamicin. The PCR analysis results indicated that the qnrA, qnrB, and qnrS genes were identified in 11 (39%), 7 (25%), and 21 (75%) of the A. baumannii isolates, respectively.
N. Tuwaij· Al-Kufa University Journal f...· 0 citations
There was a high prevalence of CR-GNB among septicemia patients, emphasizing the need for strict infection control measures to prevent their spread, and the need for strict infection control measures to prevent their spread.
Muhammad Rizwan, M. Rasool, S. Muzammil et al.· Future Microbiology· 0 citations
BACKGROUND/AIMS
Acinetobacter baumannii is an opportunistic gram-negative pathogen and an increasingly important cause of hospital-acquired infections, particularly in intensive care units. Its remarkable ability to rapidly acquire resistance mechanisms, especially against carbapenems, represents a major public health concern. This study aimed to investigate the molecular detection and characterization of OXA-type carbapenemase genes in A. baumannii isolates collected from various clinical sources in Baghdad, Iraq.
METHODS
Between March and July 2025, 36 non-repetitive A. baumannii isolates were obtained from patients with different infections. Identification was performed using standard biochemical tests, CHROMagar Acinetobacter, and the VITEK 2 system and was confirmed by PCR amplification of the intrinsic blaOXA-51 gene. Antimicrobial susceptibility testing was conducted according to CLSI guidelines. The prevalence of blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58 genes was determined by PCR. Selected PCR products were sequenced and subjected to phylogenetic analysis.
RESULTS
Extensive antimicrobial resistance was observed among the isolates, particularly to carbapenems, with resistance rates of 83.3% for imipenem and 72.2% for meropenem. High resistance rates were also detected for fluoroquinolones and aminoglycosides, whereas colistin and tigecycline retained comparatively greater activity. PCR screening revealed prevalence rates of 100% for blaOXA-51, 86.1% for blaOXA-23, 69.4% for blaOXA-24, and 47.2% for blaOXA-58. Multiple blaOXA genes were detected in more than half of the isolates, suggesting horizontal gene transfer and local clonal expansion. Phylogenetic analysis demonstrated high similarity between local isolates and international reference strains, supporting the widespread dissemination of resistance determinants. Several nucleotide substitutions were identified within the blaOXA-23 and blaOXA-24 genes.
CONCLUSION
The findings indicate that blaOXA-23 is the predominant contributor to carbapenem resistance among A. baumannii isolates in Baghdad, while blaOXA-24 and blaOXA-58 are also increasingly prevalent. The observed resistance patterns and phylogenetic relationships underscore the importance of continuous molecular surveillance, antimicrobial stewardship, and effective infection control measures to limit the spread of multidrug-resistant A. baumannii. These data contribute valuable regional information to the global understanding of antimicrobial resistance epidemiology.
A. A. Abdulhassan, Hiba Hazim Hamid, Sara Mahdi Al-Lami et al.· Cellular Physiology and Bioc...· 0 citations
The emergence of multi-drug resistant oligoclonal population of this pathogen in a hospital warrants for development of appropriate antibiotic policies and immediate implementation of infection prevention and control measures.
This study substantiates an escalated incidence of carbapenem-resistant, MDR A. baumannii in post-surgical infections in Pakistan, highlighting the exigency for strengthened antimicrobial stewardship and infection control strategies in the hospitals of the region.
Sana Gul, Nawab Ali, M. Qasim et al.· Antibiotics· 0 citations
