Skip to content
Log in
Sponsored Artificial Intelligence: A Modern Approach, Global Edition Artificial Intelligence: A Modern Approach, Global Edition

Full-length single-cell spatial transcriptomics reveals spatial and cell-type-specific transcript isoforms in the primate brain

Hengxin Liu Yanhong Hong Y. Zhang Liyuan Xi Huiying Yan Yuxuan Liu Qianqian Yang Xing Sun Shouliang Guan Zan Chen Yu Feng Tao Zeng Juan Meng Sha Liao Nini Yuan Z. Liu Chao Li Zhiyong Liu Lei Han Zhi-Jian Shen Ao Chen Yi-Di Sun Yu-Liang Dong Longqi Liu Chengyu T Li Wu Wei
Jul 2026 · Nature Methods · Vol 23, pp. 1854 - 1868 · 0 citations · 69 references
Medicine

TL;DR

Fullscope-seq resolved hundreds of major isoform switches across distinct brain regions and identified DTUs between superficial and deep cortical layers and showed substantial enrichment for neuropsychiatric disorder-associated genes.

DOI PubMed Semantic Scholar
View source

Similar papers

Open access Sep 2026

Spatial isoform sequencing at single-cell resolution reveals cell-type-specific spatial isoform variability in multiple brain cell types

Spatial long-read technologies are increasingly common but usually lack single-cell resolution. This leaves unanswered whether spatially variable isoforms reflect variability within one cell type or differences in region-specific cell-type composition. Here, we developed Spl-ISO-Seq2 (500-nm resolution) and accompanyin...

Lieke Michielsen, Andrey D. Prjibelski, Careen Foord et al. · 1 citation
Open access Sep 2026

Single-cell splice isoform usage reveals distinct axes of cellular identity and senescence

A cross-tissue single-cell long-read isoform atlas spanning 26 human tissues is presented and it is demonstrated that isoform usage is a structured, measurable axis of cellular identity that is distinct from gene expression.

Madhav Mantri, A. Detweiler, Jaeyoon Lee et al. · 0 citations
Open access Aug 2026

Long-read proteogenomic atlas of human neuronal differentiation reveals isoform diversity informing neurodevelopmental risk mechanisms

Deep long-read RNA sequencing and proteomics in induced pluripotent stem cell-derived cortical neurons is integrated to generate a high-resolution proteogenomic atlas of human neuron development, uncovering thousands of novel isoforms and revealing dynamic splicing changes in autism risk genes.

N. Xu, Katherine M. Rynard, E. Radley et al. · 1 citation
Review Open access Jul 2026

Single-cell long-read transcriptomics: from technologies to biological insights

This review examines the experimental and computational foundations of scLR-seq, including platform selection, library design, cell barcode and unique molecular identifier recovery, transcript discovery, and isoform quantification, and summarize emerging insights into isoform usage, alternative splicing, transcription...

Zeng-Jun Ren, Wen-Teng Liu, Jian-Hua Yin et al. · 0 citations
Open access Sep 2026

Systematic benchmarking of commercial workflows for isoform-resolved single-nucleus transcriptomics

This study provides a systematic assessment of four commercially available workflows for performing LR snRNA-seq and highlights key methodological trade-offs related to distinct library preparation strategies, thus providing practical guidance for future isoform-resolved transcriptome studies at the single-nucleus leve...

F. Köhler, Anna Delgado-Tejedor, Maik Zehnsdorf et al. · 0 citations

We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.