Wheat Biofortification for Enhancing Iron, Zinc, and Protein: The Role of Mutation Breeding

Abstract

Biofortification of wheat has emerged as a sustainable strategy to combat global micronutrient deficiencies, particularly iron (Fe) and zinc (Zn) deficiency, while simultaneously improving grain protein quality. Among available approaches, mutation breeding has gained renewed attention as a non-transgenic tool capable of generating novel genetic variability for nutritional enhancement. This review is based on a comprehensive analysis of peer-reviewed literature retrieved from major scientific databases, including Web of Science, Scopus, PubMed, and Google Scholar. Studies published between 2005 and 2025 were critically evaluated to compare the effectiveness, advantages, limitations, and future prospects of wheat biofortification approaches. This review critically evaluates the role of mutation breeding in wheat biofortification and compares its effectiveness with conventional breeding, agronomic biofortification, and genome editing technologies. Evidence from published studies indicates that gamma-induced mutant lines have achieved significant increases in grain Fe and Zn concentrations, as well as improvements in storage protein composition, without regulatory constraints associated with transgenic methods. However, variability in genetic stability, potential yield penalties, and genotype × environment interactions remain important limitations. Overall, integrating mutation breeding with advanced molecular tools and agronomic practices offers a promising strategy for developing nutrient-enriched wheat varieties and enhancing global food and nutritional security.