KIF13B is a novel regulator of ciliary TZ configuration, membrane composition and Shh signalling and it is shown that KIF13B regulates TZ membrane composition and ciliary SMO accumulation.
It is found that CKAP2-positive cilia are linked to cell cycle progression and that the conserved intrinsically disordered microtubule- binding domain of CKAP2, which is essential for microtubule polymerization and stabilization, is required for its ciliary localization.
L. E. da Silva, G. Campolina-Silva, Jenine Afani et al.· bioRxiv· 0 citations
An important role is identified for cilia and flagella associated protein 100 (CFAP100) in the transition zone assembly of the ciliary transition zone, providing insights into the physicochemical principle governing ciliogenesis.
Shuang Sun, Ke-Ke Li, Xia-Oou Sun et al.· Nature Communications· 0 citations
Dishevelled is a pivotal cytoplasmic hub protein that transmits Wnt signals to various downstream effectors to specify cell fates and behaviors during animal development. The PDZ domain of Dishevelled is dispensable for signaling to β-catenin but essential for multiple evolutionarily conserved β-catenin-independent Wnt...
M. Renko, Gonzalo J. Beitia, Trevor J. Rutherford et al.· Science Signaling· 0 citations
It is shown that Cfap410a is a proximity partner of Cby and that they act cooperatively in the hierarchy of the TZ assembly program by bridging the CEP290 and MKS transition zone modules.
A. Billon, Jennifer Vieillard, P. Priya et al.· bioRxiv· 0 citations
It is shown that, unlike mammals, zebrafish can tolerate complete loss of Stag1 from embryogenesis through to adulthood, and it is proposed that modulation of growth and signalling pathways compensates for the absence of Stag1, allowing embryonic development to proceed correctly.
Dylan M. Lynch, Anastasia A. Labudina, Sarada Ketharnathan et al.· bioRxiv· 0 citations
How microtubule (MT) growth is locally sustained within cilia remains a central unanswered question in ciliogenesis. Here we identify RAT-1 as a regulator of ciliary tubulin homeostasis and MT growth. Genetic screens in Caenorhabditis elegans show that loss of RAT-1 suppresses the toxic effects of a mutant α-tubulin an...