TL;DR
A detailed guide for designing CRISPRi circuits in bacteria, using the CRISPRlator, the first CRISPRi oscillator, as an example that illustrates the design process.
This review discusses about the mechanism followed by CRISPR CAS9 in gene editing and various other possible applications of CRISPR CAS9.
This work compares the performance of multiple KRAB domain systems, develops an updated CRISPRi-specific on-target scoring scheme, and quantitatively characterize off-target effects associated with seed-sequence patterns.
The principal barriers to CRISPR-Cas9 genome editing of bacteria are discussed, the current strategies for addressing these barriers are evaluated, and emerging approaches aimed at improving the efficiency, reliability and precision in bacterial genome engineering are highlighted.
A one-step CRISPR-based transformation protocol developed in this study enables fast genome editing workflows with minimal hands-on time and demonstrates the versatility of the modified system for this industrially important genus.
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