It is demonstrated that aging is driven by sequence-encoded base pairing that favors RNA expansion, alignment and the formation of a dense interchain interaction network, establishing RNA sequence patterning as a key determinant not only of phase separation but also of condensate aging and spatial organization.
Biomolecular condensates spatiotemporally regulate cellular biochemistry through liquid-liquid phase separation. In this Perspective, we refine the Condensate Code as a testable thermodynamic state map that integrates continuous cellular inputs, intrinsic molecular grammar and boundary conditions to govern measurable m...
Yao-Yao Hou, Ling-Yun Jing, Kan Sun et al.· Communications Biology· 0 citations
Biomolecular condensates provide dynamic environments that store and organize proteins, yet the fundamental principles determining whether condensation promotes or suppresses irreversible protein aggregation remain unclear. Here, we show that the receptor-interacting protein kinase 3 (RIPK3) RHIM domain follows distinc...
Tian-Chen Li, Brayden C. Williams, Qi Han et al.· bioRxiv· 0 citations
Many RNAs occupy multiple inter-converting structures in order to perform key biological functions. Characterizing RNA conformational ensembles is therefore critical to illuminating the mechanisms by which RNAs fold, unfold, and undergo precise structural rearrangements in response to cellular signals. However, resolvi...
Anjali R. Verma, P. Tiwary, Yun-Rui Qiu· bioRxiv· 0 citations
Abstract The Structural Maintenance of Chromosome (SMC) protein family plays a central role in higher-order genome organization through ATP-dependent DNA loop extrusion by cohesin and condensin and other processes. Whether these activities fully account for the complexity of chromosome architecture remains unknown. Her...
Jae-Won Jang, T. Higashi, M. Tang et al.· Nucleic Acids Research· 0 citations
The eukaryotic genome is organized into chromatin at multiple lengths and timescales. Liquid-liquid phase separation has recently emerged as a mechanism for the dynamic compartmentalization of chromatin. However, it remains unclear how cells can locally alter phase separation behavior to condense, decondense, and segre...
Jeremy Ernst, Beatriz Orozco Monroy, J. van Noort· Journal of Biological Chemis...· 0 citations
This work investigates how protein folding landscapes are altered inside condensates, using the protein α-helix as a model folded domain and develops a chemically specific, residue-resolution model for quantification of α-helical folding and applies it to characterize diverse helices within condensates of varying physi...
Nathaniel Hess, Jerelle A. Joseph· Journal of the American Chem...· 0 citations
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