gene editing

Delivering the Undeliverable: Rational Engineering of Membrane-Based and Synthetic Nanoparticle Platforms

Delivering therapeutic macromolecules to defined cell populations in vivo remains a central unsolved problem in biomedicine. Proteins, nucleic acids and viral vectors are cleared by phagocytes, degraded by enzymes, blocked by endothelial and interstitial barriers, and trapped in acidifying endolysosomes. Pre-existing i...

Biofortification of Tomato (Solanum lycopersicum) through GABA Technology for Enhanced Stress Tolerance and Nutritional Quality

Gamma-aminobutyric acid (GABA) technology is a promising biotechnology method for enhancing stress tolerance and nutritional quality in agricultural plants. Although tomato (Solanum lycopersicum) is a major vegetable crop consumed worldwide and is rich in vitamins, minerals, and antioxidants, tomato fruits naturally co...

Molecular dynamics simulation and QM/MM study of IscB-ωRNA complex

The CRISPR-Cas9 system's gene-editing fidelity relies on the HNH nuclease domain's ability to hydrolyze DNA via metal-ion catalysis. While IscB-ωRNA variants offer advantages for viral delivery due to their small size, their editing efficiency is much lower than Cas9. The understanding of how HNH-domain dynamics and me...

Engineering of CRISPR-BrCas12b System for Efficient Genome Editing in Thermophilic Bacteria

Adult heart failure molecular anchoring and functional mapping diverge across cardiac systems

Reproducibility archive for the study “Adult heart failure molecular anchoring and functional mapping diverge across cardiac systems.” This v1.0.0 package contains original figure-generation scripts, derived figure-source tables, and final PNG/SVG exports for Main Figures 1–5 and Supplementary Figures S1–S8, together w...

Genome-wide mapping of Cas9-induced sister chromatid exchange across single and over 200 genomic targets

CRISPR/Cas9-induced DNA double-strand breaks (DSBs) are widely used for genome engineering, yet their capacity to provoke sister chromatid exchange (SCE) and associated genome instability remains incompletely understood, in part because exchanges between identical sister chromatids leave no sequence change and are invi...

Discovery and engineering of avian R2 retrotransposons for all-RNA-mediated targeted DNA integration in human cells.

This work searched avian genomes, identified 159 avian R2 retrotransposons and characterized conserved/nonconserved sequence features in their proteins and untranslated region elements, which expands the avian R2 resource and provides additional tools for all-RNA-mediated genome editing.

CRISPR-Cas: Rewriting the Code of Life

Imagine being able to find a particular spelling mistake in the enormous instruction manual of life and correct it. This is the basic idea behind one of the most exciting technologies in modern biology: CRISPR-Cas gene editing. Our DNA contains the genetic instructions that control how cells develop and function. Chang...

Synthetic Dataset for PRIVAGEN-SHIELD: Privacy-Preserving Genomic Anomaly Detection

Dataset Description This repository contains the synthetic genomic workflow dataset generated and used for the experimental evaluation of the PRIVAGEN-SHIELD framework, “PRIVAGEN-SHIELD: Privacy-Preserving Genomic Anomaly Detection via Hierarchical Blockchain-Authenticated Merkle Trees and Local Differential Privacy.”...

Adult heart failure molecular anchoring and functional mapping diverge across cardiac systems

Reproducibility archive for the study “Adult heart failure molecular anchoring and functional mapping diverge across cardiac systems.” This v1.0.1 package contains original figure-generation scripts, derived figure-source tables, and final PNG/SVG exports for Main Figures 1–5 and Supplementary Figures S1–S8, together w...

The m6A regulatory network in plant stress adaptation: from mechanisms to breeding potential

N6-methyladenosine (m6A) is the most abundant internal modification of eukaryotic mRNA and is dynamically regulated by three classes of proteins: writers, erasers, and readers. Writers deposit methylation marks, erasers confer reversibility, and readers translate these signals into specific regulatory outcomes, collect...

Molecular dynamics simulation and QM/MM study of IscB-ωRNA complex

The CRISPR-Cas9 system's gene-editing fidelity relies on the HNH nuclease domain's ability to hydrolyze DNA via metal-ion catalysis. While IscB-ωRNA variants offer advantages for viral delivery due to their small size, their editing efficiency is much lower than Cas9. The understanding of how HNH-domain dynamics and me...