gene editing

Establishment and characterization of LNCaP-TR-BoM, a bone metastasis-derived prostate cancer cell line with an intermediate AR-attenuated phenotype

Background. Progression of prostate cancer under androgen receptor (AR) pathway-targeted therapy spans a phenotypic spectrum, from tumors that sustain AR pathway output to tumors that become independent of AR signaling. Experimental models that occupy the intermediate portion of this spectrum, in which AR is still expr...

Artificial Intelligence in genetic healthcare: toward adaptive and systems-level genomic medicine

Artificial intelligence is reshaping genetic healthcare through increasingly sophisticated approaches to variant interpretation, disease prediction, biomarker discovery, gene editing, and precision therapeutics. Yet these applications remain fragmented, often treating genomic and clinical information as static observat...

The Structure, Evolution, and Comparative Genomics of the Dendrobium officinale Mitochondrial Genome

A comprehensive characterization of the mitochondrial genome of D. officinale is presented, revealing a complex multibranched structure of 22 chromosomes totaling 639,931 bp, encoding 35 PCGs, 3 rRNA genes, and 24 tRNA genes.

Gene-Editing in Respiratory Disease: From Pre-Clinical Human Models to Future Therapeutics

Chronic lung diseases arise from complex interactions between environmental exposures and host genetic factors. However, these interactions are not fully understood, and despite decades of study, effective curative therapies remain unavailable for many patients. Recent breakthroughs in therapeutic gene-editing have bec...

Correction: Somatostatin receptor-targeted polymeric nanoplatform for efficient CRISPR/Cas9 gene editing to enhance synergistic hepatocellular carcinoma therapy

CRISPR-Cas Tools for Dissecting and Engineering Environmental Responses in Microalgae.

Microalgae are photosynthetic eukaryotes that need to maintain productivity under variable light, temperature, nutrient and CO2 conditions, yet their complex regulatory networks have made relevant engineering difficult. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas)...

EDIT-B consortium: an external pan-European validation of an innovative machine learning-based test for bipolar disorder diagnosis.

This study confirms the diagnostic performances of EDIT-B showing that A-to-I RNA editing coupled with ML methods is a reliable approach to distinguish BD from MDD, a significant advance towards precision psychiatry.

Transcription factor TBX2 is a key regulator of vascular calcification.

BACKGROUND AND AIMS Vascular calcification predicts cardiovascular mortality more strongly than traditional risk factors, yet its molecular basis remains poorly understood. The burden is greatest in chronic kidney disease (CKD), where disordered mineral metabolism accelerates arterial mineral deposition. METHODS Cros...

Engineering Oilseed Crops for Sustainable Production of Functional Fatty Acids

Plant oils are renewable sources of fatty acids with diverse biological, nutritional, and industrial applications. Our research focuses on developing plant-based production systems for two valuable types of fatty acids: hydroxy fatty acids (HFAs) and high-oleic oils. Using metabolic engineering and CRISPR/Cas9 genome e...

Engineering Oilseed Crops for Sustainable Production of Functional Fatty Acids

Plant oils are renewable sources of fatty acids with diverse biological, nutritional, and industrial applications. Our research focuses on developing plant-based production systems for two valuable types of fatty acids: hydroxy fatty acids (HFAs) and high-oleic oils. Using metabolic engineering and CRISPR/Cas9 genome e...

Reduced cellulose crystallinity in gene-edited poplar modulates structure–property relationships in cellulose nanofibrils and nanocrystals

Generation of DFNA5-deficient chickens reveals a species-specific role of pyroptosis in avian antiviral immunity

This work establishes the first germline-edited heterozygous DFNA5-mutant chicken model using primordial germ cell mediated genome editing and demonstrates that genetic disruption of DFNA5 markedly attenuates Raptinal-induced pyroptosis and reduces IL-1β and IL-18 secretion.