gene editing

Safety Challenges and Emerging Solutions in Regenerative Medicine: Tissue Engineering, Cell Therapy, and Gene Therapy.

This commentary highlights key safety concerns across major regenerative medicine categories and emerging solutions to address these risks, along with technological and regulatory approaches in development to improve product safety and consistency.

In vitro validation of a CRISPR/Cas9 guide RNA targeting COL1A1 gene by cleavage assay.

Functional validation of the gRNAs designed to target exon 2 of the COL1A1 gene is confirmed through in vitro cleavage activity and support its candidate selection for subsequent cellular studies targeting COL1A1, representing an initial step toward future knockout-style cellular models of Osteogenesis Imperfecta.

Comparative evaluation of germ cell-specific promoters identifies robust drivers of transgene expression during mouse spermatogenesis

The findings suggest that the Ldhc and Dazl promoters used in this study are robust and are expressed in testicular germ cells, which can be used to study gene regulation during spermatogenesis to understand testicular physiology and male fertility.

Emerging roles of microproteins in plant developmental regulation, stress adaptation and crop improvement

This review, primarily focuses on classical plant miPs that modulate protein activity through dominant-negative PPI, while also briefly discussed associated classes of small regulatory peptides, where they intersect functionally with miP-mediated regulation.

Introgressive hybridization fuels local adaptation in oakleaf butterflies

Hybridization may have multiple impacts on speciation and adaptation. Yet the evidence of adaptive introgression events that have historically occurred between nonradiating or established species is still lacking. The oakleaf butterflies in the genus Kallima include multiple sympatric and incipient species with a kno...

The epigenetic landscape of plant defense: Unlocking nature's toolkit for new plant protection

This review proposes epigenetic editing as a versatile and sustainable tool for unlocking the plant immune repertoire and developing disease‐resistant plants, offering a promising alternative that addresses several limitations of conventional genetic modification.

Graph-Immune-Optimization (GIO): An Integrated AI-Driven Framework for Simulation-Guided Design of CAR-T/NK Cell Therapy and CRISPR Gene Therapy in Cancer Immunotherapy

Precision nanomedicine for chemotherapy and gene therapy

Precision nanomedicine has emerged as a transformative approach in chemotherapy and gene therapy, leveraging advances in nanotechnology, materials science, and molecular biology to enhance drug delivery efficacy while minimizing off-target toxicity. Conventional chemotherapy faces challenges such as systemic toxicity,...

Genetic prioritization and donor-resolved microglial expression of GRN in Alzheimer's disease: reproducibility data and code

This archive (v1.0.0) accompanies the manuscript "Genetic prioritization and donor-resolved microglial expression of GRN in Alzheimer's disease". It contains the reproducibility bundle: analysis code with identified intermediate inputs, complete reported result families for the transcriptome-wide genetic screen (9,561-...

Genetic prioritization and donor-resolved microglial expression of GRN in Alzheimer's disease: reproducibility data and code

This archive (v1.0.0) accompanies the manuscript "Genetic prioritization and donor-resolved microglial expression of GRN in Alzheimer's disease". It contains the reproducibility bundle: analysis code with identified intermediate inputs, complete reported result families for the transcriptome-wide genetic screen (9,561-...

Resistance gene analogue identification and comparative genomic analysis of two Brassica carinata genomes

This study systematically identify and characterise RGAs in two newly assembled B. carinata genomes, revealing their genomic organisation, duplication patterns, and evolutionary relationships, thereby offering valuable genetic resources for developing disease-resistant and resilient cultivars of this emerging oilseed c...

《Nanomedicine & Biointerfaces at the Limit: Precision Delivery, Diagnostics, Sequencing, Regeneration, and Safe Human Interfaces》 is a flagship-scale research volume exploring what happens when nanoscale engineering begins to interact directly with living systems.

《Nanomedicine & Biointerfaces at the Limit: Precision Delivery, Diagnostics, Sequencing, Regeneration, and Safe Human Interfaces》 is a flagship-scale research volume exploring what happens when nanoscale engineering begins to interact directly with living systems. The central question is not simply: Can nanoparticles...