Quercetin exerts anti-fibrotic effects on vocal folds by multi-target inhibition of PI3K/AKT and MAPK signaling pathways, thereby modulating fibroblast behavior, preserving extracellular matrix integrity, and restoring key matrix components such as hyaluronic acid and elastic fibers.
Yujie Feng, Qiurong Yang, Yuanjia Hu et al.· Journal of Voice· 0 citations
Data indicate the KP.2 mRNA vaccine generates durable, cross-reactive responses against current Omicron subvariants, however, ongoing spike evolution impacts the neutralization of emerging lineages, highlighting the need for continued viral monitoring and timely vaccine updates.
Sanjeev Kumar, Lilin Lai, M. Ellis et al.· Journal of Virology· 0 citations
A comparative overview of dual experimental and computational advancements is provided and how the integration of generative diffusion models could facilitate the real-time simulation of conditional, multi-state structural ensembles across the redox proteome is highlighted.
T. Rass, Dana Reichmann, Gábor Erdős· FEBS Letters· 0 citations
This work discusses peptide grafting into hypervariable and framework loops, terminal fusion to antibody chains, Fc-peptide fusion proteins, and bioconjugation approaches, and examines examples that illustrate the importance of insertion site, peptide topology, and linker design for folding, activity, and developability.
Jinlan Wang, R. Romaniuk, Hristo L. Svilenov· FEBS Letters· 0 citations
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Optimization of UM171, a molecular glue initiating the degradation of neosubstrate HDAC1/2–CoREST–LSD1 through a multiprotein complex formed with KBTBD4, resulted in a 20‐fold improvement in glue activity, demonstrated by the stability of its ternary complex with KBTBD4‐HDAC2.
The model showing higher stability in molecular dynamics simulations and consistency with known structure–activity relationships served as template to design novel hNPS analogues, which revealed the model's inability to capture hNPS bioactive conformation, as most analogues were inactive as agonists.
Valentina Albanese, M. Argentieri, Federica Agosta et al.· ChemMedChem· 0 citations
Owing to the collateral trans-cleavage activity of Cas12a and the high peroxidase-like nanozyme activity of G4/Hemin, this method achieves the highly sensitive SERS detection of genetically modified samples without target gene preamplification.
Lin Liu, Chengxin Bao, Huimin Wang et al.· ACS Measurement Science Au· 0 citations
Improvements enabling quantification of site-specific modifications, including post-translational modifications and covalent compound-protein interactions spanning diverse pathways are described.
Steven R. Shuken, Geordon A. Frere, Charlotte R. Beard et al.· Nature Communications· 0 citations
Amyloid fibrils are implicated in a myriad of human diseases. A striking observation is that fibrils extracted from diseased tissues are characterized by a restricted set of folds unique to the specific pathology. In contrast, fibrils grown in vitro exhibit extensive structural diversity, suggesting that specific environmental and biochemical mechanisms in vivo enforce structural selectivity. Here, we combine two-dimensional infrared (2D IR) spectroscopy and cryo-electron microscopy (cryo-EM) to investigate the mechanisms governing polymorph formation in the human Islet Amyloid Polypeptide (hIAPP). We demonstrate that 2D IR can resolve populations of distinct polymorphs identified by cryo-EM, enabling rapid label-free screening of conditions prior to labor-intensive microscopy screening. We find that conditions favoring secondary nucleation, such as high protein concentration, increase polymorphic diversity. Crucially, cryo-EM reveals that formed by secondary nucleation do not structurally replicate the parent template. Finally, by selectively inhibiting secondary nucleation using the C-terminal domain of the DNAJB6 chaperone, we steer aggregation toward a monomorphic state. These findings highlight the critical role of molecular chaperones in fibril polymorph selection.
Mikołaj I Kuska, Łucja Kozicka, S. Prodhan et al.· bioRxiv· 0 citations
Findings identify pose ranking, rather than pose generation, as the major limitation of current cyclic peptide–protein complex prediction and demonstrate that complementary structural features can improve confidence-based pose selection.
Zhe Li, Ye Yuan, Kaiqiang Hu et al.· bioRxiv· 0 citations
This work introduces BEAM, a multiscale framework that learns slow collective variables from coarse-grained simulations to guide all-atom enhanced sampling to explain how vectorial secretion accelerates pertactin folding by excluding an off-pathway kinetic trap.
Lan Yang, Qing Luan, Michael C. Baxa et al.· bioRxiv· 0 citations
This work identified the key molecular determinant in the hHv1 channel that mediates cholesterol inhibition and also provided a mechanism linking the conversion between demosterol and cholesterol by DHCR24 to pH homeostasis in many cells, such as phagocytes, cardiomyocytes, neurons and microglial cells.
Shuo Han, R. Duan, Sarah Applewhite et al.· bioRxiv· 0 citations
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
