Single-cell, spatial transcriptomic and multi-omics analyses reveal endothelial-enriched RAPGEF2 in KIRC and Experimental validation links RAPGEF2 to tumor suppression and immunotherapy benefit.
Abstract
Highlights • Single-cell, spatial transcriptomic and multi-omics analyses reveal endothelial-enriched RAPGEF2 in KIRC.• RAPGEF2 independently predicts favorable survival across multicenter KIRC cohorts.• Experimental validation links RAPGEF2 to tumor suppression and immunotherapy benefit.
Clear cell renal cell carcinoma (ccRCC) is hallmarked by profound metabolic reprogramming; however, its intricate crosstalk with the tumor immune microenvironment (TIME) and its clinical ramifications remain inadequately elucidated. This study aims to systematically decipher the metabolic‐immune interplay in ccRCC thro...
Yi Peng, S. Qin, Xi-Wen Li et al.· Cancer Reports· 0 citations
Highlights • A macrophage-associated prognostic signature was developed for KIRC.• Prognostic genes were identified through Cox analysis and multi-algorithm screening.• An MRCS classifier was independently constructed using SLC11A1 and IFI30.• Survival stratification and validation were systematically performed.
Highlights • FABP4 is broadly downregulated across five gynecologic malignancies.• Promoter hypermethylation is associated with reduced FABP4 expression.• High FABP4 marks poor ovarian/cervical but favorable endometrial outcomes.• Single-cell and spatial data link stromal FABP4 to metabolic–immune crosstalk.• FABP4 kno...
Highlights • Single-cell analysis maps lactylation activity across the LUAD ecosystem.• Lactylation-high states show remodeled cell-cell communication networks.• A machine learning-derived LRPS predicts prognosis across cohorts.• Low LRPS is associated with greater immunotherapy benefit.• KRT7 promotes malignant LUAD p...
Meng Wang, Han Zhang, Qiuqiao Mu et al.· Translational Oncology· 1 citation
Highlights • Single-cell and machine learning defined macrophage-driven LIHC subtypes.• LGALS1 was identified as the top SHAP-ranked therapeutic evasion driver.• LGALS1 was enriched in macrophage and fibroblast lineages in LIHC.• LGALS1 silencing impaired HCC growth, migration, invasion, and clonogenicity.
Yi Wan, Qiang Huo, Yi-Jie Yang et al.· Translational Oncology· 0 citations
