2026· Current Topics in Developmental Biology· Vol 170, pp.
199-235
· 0 citations
Medicine
TL;DR
This chapter examines how 3C technologies have refined the understanding of genome organization and gene regulation and supports a model in which genome folding is governed by the biophysical properties of chromatin.
This chapter presents a step-by-step guide to ChAseR, an R package to analyze chromatin architecture using network-based approaches, and illustrates the workflow on a promoter-centered chromatin network derived from human monocytes.
Benoît Aliaga, Flavien Raynal, Vera Pancaldi· Methods in molecular biology· 0 citations
This chapter describes an optimized in situ Hi-C protocol tailored for cancer cell lines using MboI digestion and biotin-mediated pull-down to generate high-complexity libraries and outlines a computational workflow that extends beyond standard topological mapping of compartments and topologically associating domains t...
A comprehensive synthesis of the mechanisms and functional roles of classical chromatin remodelers within both physiological and neoplastic contexts is provided and a hierarchical framework that distinguishes between 'first-level' and 'higher-order' chromatin remodeling is proposed.
This study provides a resource for redefining precision-breeding paradigms by harnessing cryptic 3D chromatin targets by decoupling sequence-level from chromatin-level selection and shifting the subgenome expression balance of 39 homoeologs in cultivated cotton.
Xian-Hui Huang, Ya-Bing Peng, Xiu-Bao Hu et al.· Cell· 0 citations
It is found that, although compartments and topologically associating domains (TADs) are largely maintained, regeneration is accompanied by reduced compartmentalization and decreased boundary insulation, and 3D chromatin loops with increased contact frequency during regeneration are identified.
Palmira Llorens-Giralt, Carlos Camilleri-Robles, Leo Zuber et al.· Science Advances· 0 citations
R-loops are evolutionarily conserved three-stranded nucleic acid structures formed when a nascent RNA transcript hybridizes with its complementary DNA strand, thereby exposing the non-template strand as single-stranded DNA. Traditionally viewed as a by-product of transcription, R-loops are now recognized as critical re...
Prashant K. Mishra, M. Basrai· Open Biology· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
