Integrated Genomics and Phenotypic Analysis of Pediococcus pentosaceus BGI-N8 and Pediococcus acidilactici BGI-N9: Partial Evidence Suggesting In Vitro Probiotic Properties to Glycolipid Metabolism Regulation Potential

An integrated genomic and phenotypic insight into safety performance and probiotic potential of a novel strain Pediococcus pentosaceus K6 derived from Tibetan kefir

Pediococcus pentosaceus (P. pentosaceus) is a ubiquitous lactic acid bacterium recognized for enhancing the sensory properties, dietary benefits, and safety of fermented foods. This study isolates and characterizes a Tibetan kefir-derived strain, P. pentosaceus K6, using integrated genomic and phenotypic approaches. Whole-genome sequencing and in vitro assays confirmed a favorable safety profile for K6, showing no hemolytic activity or harmful metabolite production, and lacking pathogenic virulence determinants, transferable antibiotic resistance genes, or functional plasmid conjugation elements. K6 showed strain-specific probiotic features, including 83.83% survival at pH 3.0 for 2 h, 71.16% survival under simulated gastrointestinal digestion, 89.30% auto-aggregation at 48 h, and strong inhibition against Listeria monocytogenes (29.83 ± 0.06 mm). Compared with previously reported P. pentosaceus strains, K6 uniquely combines gastrointestinal tolerance, adhesion-related traits, and anti-Listeria activity. The antiSMASH analysis identified putative type III polyketide synthase and terpene gene clusters, providing genetic candidates for this antimicrobial phenotype. Furthermore, specific carbohydrate-active enzymes and exopolysaccharide biosynthetic genes indicate strong potential for carbohydrate metabolism and food matrix adaptation. Collectively, these genomic and phenotypic findings support P. pentosaceus K6 as a highly promising candidate for probiotic and biopreservation applications.

Complete Genome Sequence Analysis, Probiotic Properties, and Safety Assessment of Healthy Human-Derived Pediococcus pentosaceus.

Complete genome sequence of Lacticaseibacillus rhamnosus B34-0-2 isolated from Panax ginseng in South Korea.

These genome data provide a genomic resource for future studies on functional characterization and probiotic-related properties and provide a genomic resource for future studies on functional characterization and probiotic-related properties.

Whole-genome sequencing and characterization of Pseudomonas stutzeri P1 endophyte isolated from potato unveils plant growth-promoting and other traits

The potential of P1 as a promising bioinoculant candidate for sustainable agriculture in the potato sector is demonstrated and the genome lacked major virulence factors and antimicrobial traits, supporting the non-pathogenic nature of the P1 strain.

Phenotypic and genomic insights into Stenotrophomonas sepilia SMBL8: metabolic versatility, antibiotic resistance and pathogenic potential

The findings address an existing knowledge gap and highlight the potential dualistic nature of S. sepilia SMBL8, both as a beneficial industrial bacterium and an opportunistic pathogen, facilitated by metabolic versatility and genomic plasticity.

Whole-genome sequencing coupled with functional and safety validation reveals the probiotic and antimicrobial potential of traditional curd–derived Lactiplantibacillus plantarum