Porous silicon (PSi) biosensors offer significant potential for point-of-care diagnostics, yet their analytical performance is constrained by slow analyte diffusion into the porous matrix. To address this issue, we developed a lab-on-a-chip (LOC) device that combines a PSi immunosensor with a surface acoustic wave (SAW)-driven mixer, housed in a 3D-printed microfluidic flow cell. The platform was evaluated for the optical detection of lactoferrin (LF), a protein biomarker relevant to inflammatory bowel disease and chronic pancreatitis. Acoustic streaming generated by the SAW mixer enhanced convective mixing at the microscale and increased mass transfer of the target analyte towards the sensor surface. SAW-driven mixing achieved a 3-fold increase in the signal-to-noise ratio and a 5-fold reduction in the limit of detection compared to unmixed conditions, advancing the analytical range towards clinically significant concentrations.
Jonathan Nyenhuis, Josef Zürn, A. Lazanas et al.· RSC Advances· 0 citations
This strategy provides a rapid, selective, and scalable way to introduce aromatic PTMs, producing homogeneous protein libraries essential for mechanistic studies, biomarker development, and exploring the therapeutic potential of targeting aSyn-phosphorylation for PD and related disorders.
Shaswati Mandal, Xiao-Yuan Lin, Anastasia V. Gemelli et al.· Journal of the American Chem...· 0 citations
Results identify Cryaa as a critical regulator of ER stress and demonstrate that its reduction promotes UPR activation and subsequent photoreceptor apoptosis in the rd9 model, revealing a key role for Cryaa in XLRP pathology and may provide a novel therapeutic perspective for this disease.
Mingzhu Yang, Ruiqi Qiu, S. Yao et al.· Cell Death & Disease· 0 citations
ABSTRACT Microplastics (MPs) accumulation in ecosystem and human organs poses urgent environmental and health risks, yet few enzymes efficiently degrade polyethylene terephthalate (PET) under physiological conditions. We leveraged deep learning to mine unexplored sequence space across 246 million proteins, discovering AhPETase, an evolutionarily distinct hydrolase with low homology (<50% sequence identity) to known PET‐degrading enzymes. This noncanonical biocatalyst efficiently depolymerizes PET at 37°C, outperforming all typical PETases and achieving a 7.76‐fold enhancement over IsPETase, one of the most representative mesophilic PETases. Additionally, engineered variant AhPETaseM1 retains functional activity for over 20 days under physiological conditions and can degrade post‐consumer PET MPs 34‐fold faster than recombinant human‐derived enzyme MG8 (rMG8) under equal enzyme loading. Critically, it reversed PET‐induced toxicity in human lung and colon cells, establishing the first proof‐of‐concept for enzymatic MPs detoxification.
Yuxuan Wang, Shijie He, Yuheng Chang et al.· Advancement of science· 0 citations
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SIRT1–GAPDH signaling represents a post-translational axis linking sirtuin activity directly to glycolytic enzyme function, distinct from SIRT1's traditional transcriptional roles and serving as a viable molecular checkpoint in microglial immunometabolism.
Chou-Yi Hsu, I. Sapaev, O. Nematov et al.· Frontiers in Immunology· 0 citations
The results suggest that roles of CetZ1 in cell shape or potentially the organisation and structure of the cell poles influence the polar assembly of the motility machinery.
Hannah J. Brown, Md Imtiazul Islam, J. Ruan et al.· Molecular Microbiology· 0 citations
Structural and biochemical studies have elucidated the architecture of the nsp14 N7-methyltransferase domain, revealing an S-adenosyl-L-methionine (SAM)-dependent fold with a defined cofactor-binding site and an adjacent cap-binding pocket that orients the RNA substrate for methyl transfer.
Yifan Zhao, Rhea Guo, Yang Yang et al.· Microorganisms· 0 citations
The temporal decline of circulating miR-1-3p levels and the identification of PKC support a model in which miR-1-3p may contribute to functional maturation of the ZR through modulation of non-canonical intracellular signaling pathways.
Jani Liimatta, Emre Murat Altınkılıç, Therina du Toit et al.· Journal of Clinical Endocrin...· 0 citations
The data demonstrate that MRO is poised to play a role in the coordination of periovulatory events through the EGF and progesterone signaling pathways through the EGF and progesterone pathways.
Ketan Shrestha, Patrick R. Hannon, M. Wynn et al.· Biology of Reproduction· 0 citations
A cheap density prior is introduced over natural protein activations and keeps only the candidates that remain typical under it, a training-free post-hoc step the authors call Mahalanobis filtering that improves both the property score and the structural plausibility of the sequences it keeps at negligible cost, without touching the generator, and transfers across different guidance methods.
Shuibai Zhang, Xinchi Liu, Fred Zhangzhi Peng et al.· 0 citations
3D models of the C-terminal SC region suggest that the major C-terminal domain of SC folds into a single-layer β-sheet structurally similar to the membrane occupation and recognition nexus (MORN) family of tandem repeats, which is critical for understanding SC-mediated fibrin generation.
Pablo Fuentes-Prior, A. Maddur, Peter Panizzi et al.· Biological chemistry· 0 citations
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
