This study investigated the synergistic effects of supercritical carbon dioxide (SCCD) combined with sucrose treatment on the foaming properties and freeze-thaw stability of egg white protein (EWP). The results demonstrated that SCCD-sucrose treatment significantly enhanced the foaming capacity, achieving a maximum of 139.5% (4.6-fold increase over the control) at 9 MPa for 60 min with 10 g/100 mL sucrose, while maintaining the foaming stability (FS) which was compromised by SCCD treatment alone. Furthermore, the treated EWP exhibited markedly improved stability against repeated freeze-thaw cycles. Mechanism analysis revealed that sucrose promoted the formation of larger protein aggregates, as evidenced by increased particle size, and significantly reduced surface tension, enhancing adsorption at the air-water interface. Fourier transform infrared spectroscopy indicated a rise in α-helix relative content, contributing to structural ordering, while rheological measurements showed improved elastic modulus (G′), supporting FS. Scanning electron microscopy further revealed a more cohesive and dense protein network of SCCD-sucrose treated group. These synergistic modifications counteracted the structural loosening induced by SCCD and facilitated the formation of a robust interfacial film. The findings provide an effective and promising physical modification strategy for enhancing the functional performance of frozen-stored egg white liquid in the egg processing industry.
Lixian Ding, Minquan Xia, Xinyue Zhang et al.· Food Science of Animal Produ...· 0 citations
A divergent regulation of CD29 and CD49b integrins associated with HLA-B27 status in Iraqi AS patients is suggested and longitudinal validation is required to confirm their clinical utility as diagnostic or therapeutic targets.
Ahmed Ali Alshammari, M. Mohsin· Iranian Journal of Pathology· 0 citations
This is the first study reporting the use of phage/bacterium system and aggregation induced emission luminogen for detection of antibiotic residues in food sample.
Su Han Wang, Ning Ma, Jian Ping Wang· Analytica Chimica Acta· 0 citations
This study provides the first comparative transcriptomic, structural, and functional characterization of AMPs and anti-microbial proteins in N. quadripunctatus and N. littoralis, offering important foundational insights for the future development of bacteria-specific AMPs as alternatives to conventional antibiotics.
Jong Hyeok Lee, W. Kim, Yue Gao et al.· Comparative biochemistry and...· 0 citations
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This is the first study that provides direct in vitro evidence of Don induced cell cycle arrest at G2/M phase through highly upregulated p21, with no increase in p53 protein levels, suggesting p53-independent p21 induction.
Inji Shikhaliyeva, G. Albayrak, Cenk Kığ et al.· Toxicology· 0 citations
Due to its importance and wide adoption, wheat cultivation is promptly required to shift towards sustainable practices, reducing the dependency on chemical components. Among bio-based solutions aimed at securing the sustainability of wheat cultivation, biostimulants offer a versatile platform of eco-friendly tools assuring sustainability and profitability. Microalgae present a concrete example of a biostimulant source due to their richness in metabolites and high value products. Therefore, this study evaluated the biostimulant potential of eleven eco-extracts prepared from soil-isolated microalgae strains. Eco-extracts applied via soil drench at low dose (0.1 g/L) were investigated for their biostimulant effects on wheat growth, physiology, yield, and quality under controlled conditions. Results demonstrated significant ameliorations in treated plants as compared to the control, with no phytoinhibitory effects. Remarkable enhancements were notable in growth parameters such as shoot and root lengths (+40-70%), physiological traits such as total chlorophyll and stomatal conductance (+7-52%), yield components in the example of grain number per spike and thousand grain weight (+17-103%), and grain quality namely protein and polyphenol content (+2-fold to 4-fold). Similarly, phosphorus accumulation and uptake were significantly improved, while soil physicochemical status was ameliorated, indicating enhanced fertility. Multivariate analysis and composite index ranking marked Chlorella sp. GA18, Chlorella sp. GA65, Scenedesmus sp. GA69, and Chlorococcum sp. GA63 as eco-extracts with consistent performances across all plant traits. These findings highlighted the promising potential of integrating microalgae-based eco-friendly extracts in sustainable wheat cultivation.
Amer Chabili, Z. Hakkoum, F. Minaoui et al.· Plant Science· 1 citation
Control electrical stimulation significantly enhanced mucus secretion under optimized parameters of 10 V and 15 cycles, resulting in a 3.59-fold increase in mucus-associated protein output and clear electrophoretic protein profiles.
Xing Lu, Yong Long, Xiaozhen He et al.· Comparative Biochemistry and...· 0 citations
Evaluating the effectiveness of embeddings from five Protein Language Models, including ProtBERT-BFD, ESM-2, ProtALBERT, ProLLaMA, and ProtGPT-2, as input features for various machine learning classifiers suggests that while current embeddings offer strong performance, further advancements in feature extraction and model architectures are needed to significantly boost strict accuracy.
Karthik Avinash, S. Tejas, Sriram Mamidala et al.· Analytical Biochemistry· 0 citations
This study constructed a pH-responsive P-TN/SF@Fe-Cur composite coating that demonstrated significant anti-infective, anti-inflammatory, antioxidant, pro-angiogenic, and pro-osteogenic effects in rat subcutaneous infection and femoral defect models.
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
