Sep 2026· Proceedings of the National Academy of Sciences of the United States of America· Vol 123· 0 citations· 53 references
Medicine
TL;DR
A natural homolog of T7 RNA polymerase, Njord RNA polymerase, is reported on, which supports high-yield RNA synthesis at substantially lower temperatures and is a promising candidate for improving the performance and scalability of mRNA therapeutics.
Abstract
Significance Bacteriophage T7 RNA polymerase (T7 RNAP) has enabled the development of mRNA vaccines and remains central to the manufacture of this therapeutic modality. Here, we report on a natural homolog of T7 RNA polymerase, Njord RNA polymerase, which supports high-yield RNA synthesis at substantially lower temperatures. Njord RNAP generates mRNA with markedly reduced levels of inflammatory RNA contaminants, and comparative analysis of transcription reactions provides insight into the origins of these impurity species. Together, these features position Njord RNA polymerase as a valuable tool for low-temperature RNA synthesis and a promising candidate for improving the performance and scalability of mRNA therapeutics.
It is proposed that MraW modification of 16S rRNA enhances translation efficiency in general, and that specific transcripts have evolved structural features that fine-tune protein levels that may be prevalent in bacteria which exhibit uncoupled transcription and translation.
Zachory M. Park, Christina R. Savage, Amanda R. Decker-Farrell et al.· Cell Reports· 0 citations
Transfer RNAs (tRNAs) are key molecules that deliver amino acids to the translating ribosome according to their cognate codon encoded in messenger RNA (mRNA). Due to the modified nature of tRNA nucleotides, accurate tRNA quantification can be tedious, especially when dealing with small sample inputs. Here, we took adva...
É. Teyssonnière, M. Mito, Yuichi Shichino et al.· bioRxiv· 0 citations
This protocol enables effective cell lysis, nucleic acid purification, and efficient removal of genomic DNA while preserving all transcript classes, including small regulatory RNAs (sRNAs), and provides a basis for future studies investigating the gene expression dynamics and regulatory networks of F. nucleatum under d...
Falk Ponath, Jörg Vogel· Methods in molecular biology· 0 citations
Oxford Nanopore Technology (ONT) direct RNA sequencing enables the sequencing of native RNA molecules without cDNA conversion. The long-read approach captures full-length reads spanning entire genes and has transformed the study of gene expression in Plasmodium parasites by enabling analysis of untranslated regions, is...
Janne Grünebast, David Serre· Trends in Parasitology· 0 citations
Circular RNAs (circRNAs) are generated by backsplicing of eukaryotic protein-coding transcripts and can regulate microRNAs and RNA binding proteins, or serve as translation templates. Their covalently closed structure confers resistance to exonuclease-mediated degradation, extending their half-life and supporting their...
Christopher J. Fields, Rina Fujiwara, Bradley W. Wright et al.· bioRxiv· 0 citations
Two complementary methods for tsRNA validation are presented, small RNA northern blotting, which allows direct visualization of RNA size and abundance, and dumbbell PCR, which enables isoform-specific quantification with single-nucleotide resolution, which provide robust tools to validate tsRNA expression and facilitat...
Chankyung Kim, Mark A. Kay, H. Kim· Methods in molecular biology· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
